Isolation and Characterization of Pepsin-Soluble Collagen from Abalone (Haliotis discus hannai) Gastropod Muscle Part II

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著者

    • DONG Xiuping
    • School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
    • YUAN Qixin
    • School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
    • QI Hang
    • School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
    • YANG Jingfeng
    • School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
    • ZHU Beiwei
    • School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
    • ZHOU Dayong
    • School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture
    • MURATA Yoshiyuki
    • Department of biological resources chemistry, faculty of agriculture, Okayama University
    • YE Wenxiu
    • School of Food Science and Technology, Dalian Polytechnic University, Engineering Research Center of Seafood of Ministry of Education, Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture

抄録

Abalone (<i>Haliotis discus hannai</i>) gastropod muscle was divided into Part I (the center part of gastropod muscle) and Part II (the transforming and virginal part of gastropod muscle). Pepsin-soluble collagen was extracted from abalone gastropod muscle part II (PSCAGM-II) with yield of 8.7%. SDS-PAGE and amino acid composition indicated that the PSCAGM-II contained α<sub>1</sub> and α<sub>2</sub> chains and might be classified as type I collagen. PSCAGM-II exhibited a maximum absorbance at 232 nm, but little absorbance near to 280 nm. FTIR investigations showed the existence of helical arrangements of collagen.  PSCAGM-II triple helical stereochemical structure was destroyed when the treated temperature up to 35°C for 10 min. Denatured temperature detected by the viscometer was 22.9°C when incubated for 20 min. Study on the properties and thermal stability of PSC from abalone gastropod muscle has certain directive significance for the abalone processing industry.

収録刊行物

  • Food science and technology research

    Food science and technology research 18(2), 271-278, 2012-03-01

    公益社団法人 日本食品科学工学会

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各種コード

  • NII論文ID(NAID)
    10030918150
  • NII書誌ID(NCID)
    AA11320122
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13446606
  • データ提供元
    CJP書誌  J-STAGE 
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