Conformation of Single-Stranded DNA in Aqueous Solution

• TAMADA Keiko

  Department of Macromolecular Science, Osaka University

• YAMAMURA Hiroki

  Department of Macromolecular Science, Osaka University

• TERAO Ken

  Department of Macromolecular Science, Osaka University

• SATO Takahiro

  Department of Macromolecular Science, Osaka University

Bibliographic Information

Other Title

• 一本鎖DNAの水溶液中での分子形態
• イッポンサ DNA ノ スイヨウエキ チュウ デ ノ ブンシ ケイタイ

Abstract

The conformation of thermally denatured single-stranded DNA was studied by size exclusion chromatography equipped with a multi-angle light scattering on-line detector (SEC-MALS) and small angle X-ray scattering (SAXS). A sonicated sodium salt DNA sample from salmon testes dissolved in 0.015 M aqueous NaCl was kept at 85°C for 10 min, and then cooled to 10°C at different ionic strengths before SEC-MALS and SAXS measurements. The results were analyzed using the wormlike chain or wormlike cylinder model to determine the contour length per nucleotide unit, the persistence length representing the polymer chain stiffness, and the chain thickness. The parameters obtained indicate that the single-stranded DNA chain is a typically flexible polyelectrolyte, of which conformation does not take the fully extended conformation but locally helical conformation.

Journal

• KOBUNSHI RONBUNSHU

  KOBUNSHI RONBUNSHU 69 (7), 399-405, 2012

  The Society of Polymer Science, Japan

Keywords

• Single-Stranded DNA
• SEC-MALS
• SAXS
• Wormlike Chain
• Polyelectrolyte

Details

• CRID: 1390282681503302272
• NII Article ID: 10030932372
• NII Book ID: AN00085011
• DOI: 10.1295/koron.69.399
• COI: 1:CAS:528:DC%2BC38XhtFegs7nP
• ISSN: 18815685
• ISSN: 03862186
• NDL BIB ID: 023905773
• Web Site: http://id.ndl.go.jp/bib/023905773
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023905773
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed