Local Transplant of Human Umbilical Cord Matrix Stem Cells Improves Skin Flap Survival in a Mouse Model

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著者

    • LENG Xiangfeng
    • Department of Plastic Surgery, The Affiliated Hospital of Medical College Qingdao University
    • ZHANG Qiu
    • School of Chemistry and Chemical Engineering, Shandong University
    • ZHAI Xiaodong
    • Department of Plastic Surgery, The Affiliated Hospital of Medical College Qingdao University
    • CHEN Zhenyu
    • Department of Plastic Surgery, The Affiliated Hospital of Medical College Qingdao University

抄録

A skin flap is a piece of skin that has its own blood supply, which is useful to repair large skin defects and deep wounds in plastic surgery. However, partial skin flap necrosis usually occurred. Bone marrow mesenchymal stem cells (BM MSCs) are effective in improving the ischemic flap survival, but their clinical application is restricted by their limited source. Human umbilical cord matrix stem (HUCMS) cells are easily isolated in a large number, compared to BM MSCs. In this study, we evaluated the therapeutic potential of HUCMS to improve the survival of ischemic skin flap. HUCMS cells were characterized with surface markers, and were labeled with 5-acetylene base-2 'deoxidizing uracil nucleoside (EdU) in vitro. Twenty male immunodeficient BALB/c mice with an epigastric flap were randomly divided into two groups. HUCMS cells or Dulbecco's Modified Eagle Medium (DMEM) were injected into the subcutaneous flap tissues. On the 7th postoperative day, flap survival, capillary density, levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), and EdU-positive cells in the skin flap were examined. Results showed that flap survival rate was higher in the HUCMS cell group (<i>P</i> < 0.05). Capillary density, VEGF level, and bFGF level were higher in the HUCMS cell group (<i>P</i> < 0.05). EdU-labeled HUCMS cells were mainly distributed in the subcutaneous flap tissues. These findings suggest that HUCMS cells can improve the survival of ischemic skin flap by promoting vascularization, which may be attributed to the increased expression of VEGF and bFGF.

収録刊行物

  • Tohoku journal of experimental medicine

    Tohoku journal of experimental medicine 227(3), 191-197, 2012-07-01

    Tohoku University Medical Press

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各種コード

  • NII論文ID(NAID)
    10030943777
  • NII書誌ID(NCID)
    AA00863920
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00408727
  • データ提供元
    CJP書誌  J-STAGE 
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