アミノ基の高速化学標識に基づくN-結合型糖鎖の非侵襲的イメージングとターゲティング : 糖タンパク質、デンドリマー、および生細胞上のN-結合型糖鎖の機能解析を目指して  [in Japanese] Development of Azaelectrocyclization-Based Labeling and Application to Noninvasive Imaging and Targeting Using N-Glycan Derivatives : In Pursuit of N-Glycan Functions on Proteins, Dendrimers, and Living Cells  [in Japanese]

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Abstract

著者らは、独自に開発した高速6π-アザ電子環状反応を基盤として、リジン側鎖のアミノ基にポジトロン放出核種や蛍光基で標識する新規方法を開発した。また、同反応を用いて細胞表層上のアミノ基に対して穏やかな条件下で簡便に有機低分子を導入することにも成功し、細胞表層を化学的に複合型<I>N</I>-型糖鎖で修飾する化学的手法を確立した。これらの方法を用いて、糖タンパク質や生体内での糖鎖構造を疑似化した世界最大のクラスターのPETや非侵襲的な蛍光イメージングを実施することにより、<I>N</I>-型糖鎖の非還元末端シアル酸の存在やガラクトースとの結合様式に依存する動態や代謝過程の相違を初めて可視化することに成功した。さらに、リンパ球の表面に対して有機合成化学的に複合型糖鎖を導入することにより、生きた動物内で癌を認識させることに成功した。

The authors have developed the new positron emitting metal and fluorescence labeling protocol of ε-amino group of lysine through their rapid 6π-azaelectrocyclization. Using the same reaction, the small organic molecules could also be introduced on the amino groups of the living cell surface with simple operations under quite mild conditions, establishing the chemistry-based cell surface engineering with complex-type <I>N</I>-glycans. PET and the noninvasive fluorescence imaging of the <I>N</I>-glycoproteins and the world’s largest <I>N</I>-glycoclusters, mimicking the natural <I>glyco-bioenvironment</I>, for the first time visualized the differences in their <I>in vivo</I> dynamics and excretion pathway depending on the presence of the non-reducing end sialic acid as well as their linkages to the galactose residue. The authors also synthetically developed the artificial lymphocytes to target the tumors in living animals by introducing the complex-type <I>N</I>-glycans on the cell surfaces.

Journal

  • Trends in Glycoscience and Glycotechnology

    Trends in Glycoscience and Glycotechnology 24(135), 47-64, 2012-05-31

    FCCA(Forum: Carbohydrates Coming of Age)

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Codes

  • NII Article ID (NAID)
    10030975689
  • NII NACSIS-CAT ID (NCID)
    AA10995236
  • Text Lang
    JPN
  • Article Type
    REV
  • ISSN
    09157352
  • NDL Article ID
    023936905
  • NDL Call No.
    Z17-1303
  • Data Source
    CJP  NDL  J-STAGE 
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