No Involvement of Lysophosphatidic Acid Receptor-3 in Cell Migration of Mouse Lung Tumor Cells Stimulated by 12-O-Tetradecanoylphorbol-13-acetate

• Okumura Mai

  Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University

• Kato Kohei

  Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University

• Fukui Rie

  Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University

• Fukushima Nobuyuki

  Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and Engineering, Kinki University

• Tsujiuchi Toshifumi

  Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University

Abstract

The tumor promoting agent 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulates cell migration of several tumor cells. Recently, we reported that loss of lysophosphatidic acid (LPA) receptor-3 (LPA₃) enhanced cell migration of murine lung tumor LL/2 cells. In the present study, we investigated whether LPA₃ is involved in cell migration of mouse lung tumor cells stimulated by TPA. Exogenous LPA₃ gene (Lpar3)-expressing (LL/2-a3) cells and LL/2-AB cells as a vector control generated from LL/2 cells were used. In a cell migration assay, TPA treatment significantly stimulated cell migration of LL/2-AB and LL/2-a3 cells, while the cell migration abilities of LL/2-a3 were markedly lower than those of LL/2-AB cells. Using quantitative real-time reverse transcription (RT)-polymerase chain reaction (PCR) analysis, no effect of TPA treatment on the expression levels of LPA₁, LPA₂ and LPA₃ genes was detected in either type of cells. These results suggest that the LPA₃ may not be involved in the enhanced migration ability by TPA in mouse lung tumor cells.

Journal

• Journal of Toxicologic Pathology

  Journal of Toxicologic Pathology 24 (3), 183-186, 2011

  JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY

Keywords

• LPA₃
• 12-O-tetradecanoylphorbol-13-acetate
• cell migration
• lung
• mouse

Details

• CRID: 1390282679391328000
• NII Article ID: 10030980454
• NII Book ID: AN10232280
• DOI: 10.1293/tox.24.183
• ISSN: 1881915X
• ISSN: 09149198
• NDL BIB ID: 11267650
• Web Site: http://id.ndl.go.jp/bib/11267650
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11267650
• Web Site: http://www.jstage.jst.go.jp/article/tox/24/3/24_3_183/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012183866
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed