DNAがつくるソフト界面の特異な性質

• 前田 瑞夫

  独立行政法人理化学研究所　前田バイオ工学研究室

書誌事項

タイトル別名

• DNA-Based Soft Interface and Its Unique Properties
• DNA ガ ツクル ソフト カイメン ノ トクイ ナ セイシツ

抄録

We have studied unique functions of DNA-modified nanoparticles, which disperse in an aqueous medium due to electrostatic repulsion between anionic phosphate groups in the DNA backbone. Interestingly, when complementary single-stranded DNA, whose base number is identical to that of the DNA on the surface, is added to the dispersion of DNA nanoparticles to form the fully matched double helix on the surface, the DNA nanoparticles become unstable and spontaneously form aggregates in a non-crosslinking manner. Furthermore, we have found that the doublestranded DNA-carrying nanoparticles acquire high colloidal stability to disperse in an aqueous medium when a terminal single-base mismatch exists at the interface between the DNA corona and the disperse medium. Exploiting the unique colloidal behavior of the DNA nanoparticles, we have devised a facile single-nucleotide polymorphism genotyping method. We applied the SPR imaging technique on microfluidic devices to the detection of the nanoparticles aggregation.

収録刊行物

• 膜

  膜 37 (4), 183-188, 2012

  日本膜学会

キーワード

• nanoparticle
• gold nanoparticle
• poly(N-isopropylacrylamide)
• DNA
• graft copolymer

詳細情報

• CRID: 1390282681398765568
• NII論文ID: 10030986195
• NII書誌ID: AN0023215X
• DOI: 10.5360/membrane.37.183
• COI: 1:CAS:528:DC%2BC38XhtlGgtLvF
• ISSN: 18846440
• ISSN: 03851036
• NDL書誌ID: 023910369
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023910369
• Web Site: https://www.jstage.jst.go.jp/article/membrane/37/4/37_183/_pdf
• 本文言語コード: ja
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