DNA-Based Soft Interface and Its Unique Properties
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- Maeda Mizuo
- Bioengineering Laboratory, RIKEN Institute
Bibliographic Information
- Other Title
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- DNAがつくるソフト界面の特異な性質
- DNA ガ ツクル ソフト カイメン ノ トクイ ナ セイシツ
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Abstract
We have studied unique functions of DNA-modified nanoparticles, which disperse in an aqueous medium due to electrostatic repulsion between anionic phosphate groups in the DNA backbone. Interestingly, when complementary single-stranded DNA, whose base number is identical to that of the DNA on the surface, is added to the dispersion of DNA nanoparticles to form the fully matched double helix on the surface, the DNA nanoparticles become unstable and spontaneously form aggregates in a non-crosslinking manner. Furthermore, we have found that the doublestranded DNA-carrying nanoparticles acquire high colloidal stability to disperse in an aqueous medium when a terminal single-base mismatch exists at the interface between the DNA corona and the disperse medium. Exploiting the unique colloidal behavior of the DNA nanoparticles, we have devised a facile single-nucleotide polymorphism genotyping method. We applied the SPR imaging technique on microfluidic devices to the detection of the nanoparticles aggregation.
Journal
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- MEMBRANE
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MEMBRANE 37 (4), 183-188, 2012
THE MEMBRANE SOCIETY OF JAPAN
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Details 詳細情報について
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- CRID
- 1390282681398765568
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- NII Article ID
- 10030986195
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- NII Book ID
- AN0023215X
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- COI
- 1:CAS:528:DC%2BC38XhtlGgtLvF
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- ISSN
- 18846440
- 03851036
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- NDL BIB ID
- 023910369
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed