Immobilization of a Thermostable Cellobiose 2-Epimerase from <i>Rhodothermus marinus</i> JCM9785 and Continuous Production of Epilactose

• SATO Hiroki

  Research Faculty of Agriculture, Hokkaido University

• SABURI Wataru

  Research Faculty of Agriculture, Hokkaido University

• OJIMA Teruyo

  Nihon Shokuhin Kako Co., Ltd.

• TAGUCHI Hidenori

  Research Faculty of Agriculture, Hokkaido University

• MORI Haruhide

  Research Faculty of Agriculture, Hokkaido University

• MATSUI Hirokazu

  Research Faculty of Agriculture, Hokkaido University

Bibliographic Information

Other Title

• Immobilization of a Thermostable Cellobiose 2-Epimerase from Rhodothermus marinus JCM9785 and Continuous Production of Epilactose

Abstract

Cellobiose 2-epimerase (CE) efficiently forms epilactose which has several beneficial biological functions. A thermostable CE from Rhodothermus marinus was immobilized on Duolite A568 and packed into a column. Lactose (100 g/L) was supplied to the reactor, kept at 50 °C at a space velocity of 8 h⁻¹. The epilactose concentration of the resulting eluate was 30 g/L, and this was maintained for 13 d.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 76 (8), 1584-1587, 2012

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• epilactose
• cellobiose 2-epimerase
• <i>Rhodothermus marinus</i>
• immobilization
• cross-linking

Details

• CRID: 1390001206477099136
• NII Article ID: 130004137936
• NII Article ID: 10031066440
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.120284
• COI: 1:STN:280:DC%2BC38fmslOltQ%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 023916365
• PubMed: 22878201
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023916365
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.120284
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed