大脳皮質介在ニューロン移動と成熟のライブイメージング  [in Japanese] Live Imaging of Migration and Maturation of Corical Interneurons  [in Japanese]

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Abstract

<p>大脳皮質はヒトの様々な高次機能の担い手である.大脳皮質は興奮性投射ニューロンと抑制性介在ニューロンから成るが,後者の占める割合は2割程度と低いものの極めて多様性に富んでおり,高次機能発現における役割の認識が高まっている.したがって,大脳皮質の成り立ちの理解にとって如何にして抑制性介在ニューロンが大脳皮質に組み込まれていくかを明らかにすることは極めて重要である.我々は抑制性ニューロン特異的に緑色蛍光蛋白(GFP)を発現するマウス及び電気穿孔法で抑制性介在ニューロン特異的に遺伝子を導入することで皮質の抑制性ニューロンを標識し,その動態を追った.大脳基底核隆起で発生した抑制性ニューロンは脳の深部の中間帯・脳室下帯を接線方向に移動して皮質の背側部に辿り着いた後,辺縁帯で長期間に亙り酔歩用の動きを示した.これを乱すと抑制性ニューロンの脳内最終分布が変化することなどから,この動きは抑制性ニューロンが皮質に万遍なく広がるのに重要な役割を果たすものと考えられる.</p>

<p>Cerebral cortex in mammals is composed of excitatory projection neurons and inhibitory interneurons.  Due to their remarkable diversity, interneurons are thought to play important roles in emergence of higher brain functions. Thus, it is important to know how each interneuron subtypes is sorted into correct positions within the cerebral cortex. We addressed this issue by live imaging of interneurons utilizing glutamate decarboxylase (GAD) 67-GFP (green fluorescent protein) mice, in which GFP is specifically expressed by inhibitory interneurons, and <i>in utero</i> electroporation of appropriate constructs to the site of interneuron generation. We found that interneurons generated in the medial ganglionic eminence of the basal forebrain tangentially migrate to the cortex by way of the intermediate zone and the subventricular zones. These neurons then translocate to the marginal zone near the cortical surface, where they execute multidirectional tangential migration lasting for a few days.  Disruption of tangential migration of interneurons in the MZ altered the final distribution of interneurons subtypes, suggesting that this mode of migration play important roles for correct sorting of interneurons subtypes into appropriate regions within the cortex.</p>

Journal

  • KENBIKYO

    KENBIKYO 47(3), 149-156, 2012-09-30

    The Japanese Society of Microscopy

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Codes

  • NII Article ID (NAID)
    10031121399
  • NII NACSIS-CAT ID (NCID)
    AA11917781
  • Text Lang
    JPN
  • Article Type
    REV
  • ISSN
    13490958
  • NDL Article ID
    024037948
  • NDL Call No.
    Z16-896
  • Data Source
    CJP  NDL  J-STAGE 
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