Microscale thioacidolysis method for the rapid analysis of β-<i>O</i>-4 substructures in lignin

• Yamamura Masaomi

  Research Institute for Sustainable Humanosphere, Kyoto University

• Hattori Takefumi

  Institute of Socio-Arts and Sciences, The University of Tokushima

• Suzuki Shiro

  Research Institute for Sustainable Humanosphere, Kyoto University

• Shibata Daisuke

  Kazusa DNA Research Institute

• Umezawa Toshiaki

  Research Institute for Sustainable Humanosphere, Kyoto University Institute of Sustainability Science, Kyoto University

Bibliographic Information

Other Title

• Microscale thioacidolysis method for the rapid analysis of β-O-4 substructures in lignin
• Microscale thioacidolysis method for the rapid analysis of ^|^#946;-O-4 substructures in lignin

Abstract

Thioacidolysis is a method to detect the β-O-4 substructures of lignin, and has been employed as a diagnostic test for the presence of lignin. However, the conventional thioacidolysis protocol is low-throughput and is a bottleneck in the characterization of lignins in a large number of samples such as transgenic lines. Recently, a rapid analysis protocol for thioacidolysis was reported. In this study, we modified mainly the work-up process. Our microscale protocol showed higher yields of thioacidolysis products than the conventional protocol.

Journal

• Plant Biotechnology

  Plant Biotechnology 29 (4), 419-423, 2012

  Japanese Society for Plant Biotechnology

Keywords

• High-throughput
• lignin
• microscale
• thioacidolysis
• β-<i>O</i>-4 substructure

Details

• CRID: 1390282679303526400
• NII Article ID: 10031122169
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.12.0627a
• COI: 1:CAS:528:DC%2BC38XhslSnsb3I
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 023997482
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023997482
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed