Acute oral toxicity evaluation of symmetrically branched glycerol trimer in ddY mice

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Author(s)

    • KONO Mai
    • Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
    • FUJII Shoko
    • Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
    • MATSUSHITA Tsuyoshi
    • Department of Pharmaceutical Chemistry, Institute of Health Biosciences, The University of Tokushima Graduate School
    • HATTORI Hatsuhiko
    • Department of Pharmaceutical Chemistry, Institute of Health Biosciences, The University of Tokushima Graduate School
    • ISHIZAWA Keisuke
    • Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
    • NEMOTO Hisao
    • Department of Pharmaceutical Chemistry, Institute of Health Biosciences, The University of Tokushima Graduate School
    • TSUCHIYA Koichiro
    • Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School

Abstract

Lipophilic-hydrophilic balance is a quite important determinant of pharmacokinetic properties of pharmaceuticals. Thus it is a key step to successfully manage lipophilic-hydrophilic balance in drug design. We have designed unique modular molecules, symmetrically branched oligoglycerols (BGL) as an alternative means to endow hydrophobic molecules with much hydrophilicity. We have succeeded in improving the water-solubility of several hydrophobic medicinal small molecules and thermal stability of artificial protein by covalent conjugation to BGL. We have also demonstrated that a representative BGL, symmetrically branched glycerol trimer (BGL003) does not exhibit significant cytotoxicity against human hepatocarcinoma HepG2 cells. However, there have been no reports suggesting whether BGL could be used in safety in vivo. Therefore, evaluation of acute oral toxicity of BGL003 in healthy mice was conducted. Here we demonstrate that an oral administration of BGL003 did not exhibit acute lethal toxicity up to 3,000 mg/kg. Body weight, food intake, blood glucose levels and weights of tissues were not affected by a short-term repetitive administration of increasing doses of BGL003. Biochemical indications related to hepatic disorders and tissue damage were unchanged, either. A single administration study revealed that 50% lethal dose of BGL003 should be more than 2,000 mg/kg. BGL003 will be safe and suitable approach to improve hydrophilicity of hydrophobic compounds.

Journal

  • The Journal of Toxicological Sciences

    The Journal of Toxicological Sciences 37(6), 1253-1259, 2012-12-01

    The Japanese Society of Toxicology

References:  28

Cited by:  1

Codes

  • NII Article ID (NAID)
    10031126029
  • NII NACSIS-CAT ID (NCID)
    AN00002808
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    03881350
  • NDL Article ID
    024151685
  • NDL Call No.
    Z19-1022
  • DOI
    10.2131/jts.37.1253
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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