Influence of the Charge State of Precursor Ions on the Collision-Induced Dissociation of Carbonic Anhydrase 2
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- NABUCHI Yoshiaki
- Graduate School in Nanobioscience, Yokohama City University
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- HIROSE Kenji
- Technology Application Department, Nihon Waters K.K.
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- TAKAYAMA Mitsuo
- Graduate School in Nanobioscience, Yokohama City University
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Product ion spectra of bovine carbonic anhydrase 2 (CA2) were obtained, in an attempt to examine influence of charge number of precursor ions, which are produced by electrospray ionization (ESI), on collision-induced dissociation (CID). In the product ion spectra obtained from the precursor ions with various charge numbers, the observed m/z values of the product ions were common among the product ion spectra. Product ions which are comprised of complementary ion pairs (b- and y-series ions) were observed to cover the entire CA2 sequence. The sum totals of the charge number of the paired b- and y-series ions were equal to the charge number of the precursor ions. Regarding the intensity of the product ions, the charge number of the most abundant peak for b-series ions shifted in proportion as the charge number of the precursor ions, whereas that of y-series ions were fixed at 5+. The results suggest that the gas phase CA2 ions consist of the following two parts; 1) the region containing the C-terminal, which can maintain a certain folding state, and 2) the N-terminal region which was extensively altered as the result of the change in charge state. The advantageous point for product ion measurement is that the method can provide information concerning the folding state of the partial structure of proteins. Regarding the folding states of proteins, not only the dissociation position of the product ions but also the intensities of both the precursor and the product ions appear to be important.
収録刊行物
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- 質量分析
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質量分析 60 (4), 47-50, 2012
一般社団法人 日本質量分析学会
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詳細情報 詳細情報について
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- CRID
- 1390001206496762880
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- NII論文ID
- 10031126335
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- NII書誌ID
- AN0010555X
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- COI
- 1:CAS:528:DC%2BC38XhvVSnt7%2FL
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- ISSN
- 18804225
- 18843271
- 13408097
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- NDL書誌ID
- 023980735
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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- 使用不可