Flexible Xxx–Asp/Asn and Gly–Xxx Residues of Equine Cytochrome c in Matrix-Assisted Laser Desorption/Ionization In-Source Decay Mass Spectrometry
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- Takayama Mitsuo
- Graduate School of Nanobioscience, Yokohama City University
書誌事項
- タイトル別名
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- Flexible Xxx–Asp/Asn and Gly–Xxx Residues of Equine Cytochrome <i>c</i> in Matrix-Assisted Laser Desorption/Ionization In-Source Decay Mass Spectrometry
- Flexible Xxx^|^#8211;Asp/Asn and Gly^|^#8211;Xxx Residues of Equine Cytochrome c in Matrix-Assisted Laser Desorption/Ionization In-Source Decay Mass Spectrometry
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The backbone flexibility of a protein has been studied from the standpoint of the susceptibility of amino acid residues to in-source decay (ISD) in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Residues more susceptible to MALDI-ISD, namely Xxx–Asp/Asn and Gly–Xxx, were identified from the discontinuous intense peak of c′-ions originating from specific cleavage at N–Cα bonds of the backbone of equine cytochrome c. The identity of the residues susceptible to ISD was consistent with the known flexible backbone amides as estimated by hydrogen/deuterium exchange (HDX) experiments. The identity of these flexible amino acid residues (Asp, Asn, and Gly) is consistent with the fact that these residues are preferred in flexible secondary structure free from intramolecular hydrogen-bonded structures such as α-helix and β-sheet. The MALDI-ISD spectrum of equine cytochrome c gave not only intense N-terminal side c′-ions originating from N–Cα bond cleavage at Xxx–Asp/Asn and Gly–Xxx residues, but also C-terminal side complement z·-ions originating from the same cleavage sites. The present study implies that MALDI-ISD can give information about backbone flexibility of proteins, comparable with the protection factors estimated by HDX.
収録刊行物
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- Mass Spectrometry
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Mass Spectrometry 1 (2), A0007-A0007, 2012
一般社団法人 日本質量分析学会
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詳細情報 詳細情報について
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- CRID
- 1390001205415469184
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- NII論文ID
- 10031126344
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- NII書誌ID
- AN0010555X
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- ISSN
- 13408097
- 21865116
- 2187137X
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- COI
- 1:STN:280:DC%2BC2c3os1WmsQ%3D%3D
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- NDL書誌ID
- 024140145
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- PubMed
- 24349908
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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