An Assay for Carbohydrate-Binding Activity of Lectins Using Polyamidoamine Dendrimer Conjugated with Carbohydrates

• HATAKEYAMA Tomomitsu

  Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University

• KARINO Ryota

  Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University

• TERAI Yasuaki

  Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University

• KIMURA Masanari

  Organic and Bioorganic Chemistry Laboratories, Graduate School of Engineering, Nagasaki University Organic and Bioorganic Chemistry Laboratories, Graduate School of Engineering, Nagasaki University

• UNNO Hideaki

  Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University

• GODA Shuichiro

  Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University

抄録

The carbohydrate-binding activity of lectins was examined using polyamidoamine dendrimer conjugated with carbohydrates (sugar-PD). When a C-type lectin, CEL-IV, was mixed with melibiose-PD, large complexes with a diameter of about 1 μm were formed. Changes in the amount of CEL-IV/melibiose-PD complex as an indication of lectin activity were measured sensitively by Rayleigh scattering. The carbohydrate specificity of the lectin was determined on the basis of inhibition of complex-formation by individual carbohydrates. It is suggested that various lectins can also be measured using sugar-PDs to which different carbohydrates are attached.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 76 (10), 1999-2001, 2012

  公益社団法人 日本農芸化学会

キーワード

• lectin
• polyamidoamine dendrimer
• carbohydrate
• binding
• light scattering

詳細情報

• CRID: 1390282681455080960
• NII論文ID: 130004054025
• NII論文ID: 10031126497
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.120520
• COI: 1:STN:280:DC%2BC3s%2FjsFGnsA%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024036046
• PubMed: 23047090
• Web Site: http://id.ndl.go.jp/bib/024036046
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024036046
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.120520
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
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  • KAKEN
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