Enzymatic Assay for <small>D</small>-Aspartic Acid Using <small>D</small>-Aspartate Oxidase and Oxaloacetate Decarboxylase

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  • KATO Shiro
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
  • IKUTA Tomoyuki
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
  • HEMMI Hisashi
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
  • TAKAHASHI Shoji
    Department of Civil and Environmental Engineering, Nagaoka University of Technology Department of Civil and Environmental Engineering, Nagaoka University of Technology
  • KERA Yoshio
    Department of Civil and Environmental Engineering, Nagaoka University of Technology Department of Civil and Environmental Engineering, Nagaoka University of Technology
  • YOSHIMURA Tohru
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University

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  • Enzymatic Assay for D-Aspartic Acid Using D-Aspartate Oxidase and Oxaloacetate Decarboxylase
  • Enzymatic Assay for<scp>D</scp>-Aspartic Acid Using<scp>D</scp>-Aspartate Oxidase and Oxaloacetate Decarboxylase

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Abstract

An enzymatic assay system for D-Asp was established using D-aspartate oxidase and oxaloacetate decarboxylase. In this system, D-Asp is converted to pyruvate, which is determined fluorometrically with 1,2-diamino-4,5-methylenedioxybenzene. This method makes possible D-Asp measurement at the micromolar level. The D-Asp contents of an edible brown alga, Hijika fusiforme, a lactic acid bacteria beverage, and pig testis were determined by the method.

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