Development of an Analytical Method for the Determination of Arsenic in Urine by Gas Chromatography-mass Spectrometry for Biological Monitoring of Exposure to Inorganic Arsenic

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  • Takeuchi Akito
    Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association, Japan
  • Namera Akira
    Department of Forensic Medicine, Institute of Biomedical and Health Sciences, Hiroshima University, Japan
  • Kawasumi Yaeko
    Occupational Health Research and Development Center, Japan Industrial Safety and Health Association, Japan
  • Imanaka Tsutoshi
    Fukushima Factory, GL Sciences Inc., Japan
  • Sakui Norihiro
    Agilent Technologies, Japan
  • Ota Hirokazu
    Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association, Japan
  • Endo Yoko
    Research Center for Occupational Poisoning, Kansai Rosai Hospital, Japan Labour Health and Welfare Organization, Japan
  • Sumino Kimiaki
    Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association, Japan
  • Endo Ginji
    Department of Preventive Medicine and Environmental Health, Graduate School of Medicine, Osaka City University, Japan

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  • Development of an Analytical Method for the Determination of Arsenic in Urine by Gas Chromatography‐mass Spectrometry for Biological Monitoring of Exposure to Inorganic Arsenic

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Abstract

Objectives: The purpose of this study was to develop an analytical method for the simultaneous determination of inorganic arsenic [As(III) and As(V)] and monomethylarsonic acid (MMA) in urine by gas chromatography-mass spectrometry (GC-MS) for the biological monitoring of exposure to inorganic arsenic. Methods: Arsenic compounds (after reduction of arsenic to the trivalent state) were derivatized with 2,3-dimercapto-1-propanol and then analyzed using a GC-MS. The proposed method was validated according to the US Food and Drug Administration guidelines. The accuracy of the proposed method was confirmed by analyzing Standard Reference Material (SRM) 2669 (National Institute of Standards and Technology). Results: Calibration curves showed linearity in the range 1–100 μg/l for each of the arsenic species, with correlation coefficients of >0.999. For each of the arsenic species, the limits of detection and quantification were 0.2 μg/l and 1 μg/l, respectively. The recoveries were 96–100%, 99–102% and 99–112% for As(III), As(V) and MMA, respectively. Intraday accuracy and precision were 82.7–99.8% and 0.9–7.4%, respectively. Interday accuracy and precision were 81.3–100.0% and 0.8–9.9%, respectively. The analytical values of SRM 2669 obtained by the proposed method were sufficiently accurate. Conclusions: The proposed method overcame the disadvantages of high-performance liquid chromatography with inductively coupled plasma mass spectrometry. It was a robust, selective and cost-effective method suitable for routine analyses and could be useful for the biological monitoring of occupational exposure to inorganic arsenic.

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