Immobilization of Trypsin on Graphene Oxide Nanosheets for Increased Proteolytic Stability

• Masayo Sakata

  Graduate School of Science and Technology, Kumamoto University

• Asami Funatsu

  Graduate School of Science and Technology, Kumamoto University

• Shohei Sonoda

  Graduate School of Science and Technology, Kumamoto University

• Tatsuya Ogata

  Graduate School of Science and Technology, Kumamoto University

• Takaaki Taniguchi

  Graduate School of Science and Technology, Kumamoto University

• Yasumichi Matsumoto

  Graduate School of Science and Technology, Kumamoto University

抄録

To increase the thermal and chemical stabilities of trypsin-proteolysis activity, trypsin was immobilized on graphene oxide nanosheets (GONSs) by a one-step diimide-activated amidation. The trypsin-immobilized GONS (1.9 mg per mg-GONS) showed significantly higher thermal and chemical stabilities than the free form. After heat treatment at 50 °C for 6 h, the trypsin–GONS retained 80% of its initial activity, but free trypsin retained only 4% of its initial activity.

収録刊行物

• Chemistry Letters

  Chemistry Letters 41 (12), 1625-1627, 2012-12-01

  Oxford University Press (OUP)

キーワード

• General Chemistry

詳細情報

• CRID: 1360283694088365056
• NII論文ID: 10031135772
• NII書誌ID: AA00603318
• DOI: 10.1246/cl.2012.1625
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/41/12/1625/55640884/cl.2012.1625.pdf
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