Bacillus thuringiensis Suppresses Bacterial wilt Disease Caused by Ralstonia solanacearum with Systemic Induction of Defense-Related Gene Expression in Tomato

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Abstract

<i>Bacillus thuringiensis</i> is a naturally abundant Gram-positive bacterium and a well-known, effective bio-insecticide. Recently, <i>B. thuringiensis</i> has attracted considerable attention as a potential biological control agent for the suppression of plant diseases. In this study, the bacterial wilt disease-suppressing activity of <i>B. thuringiensis</i> was examined in tomato plants. Treatment of tomato roots with <i>B. thuringiensis</i> culture followed by challenge inoculation with <i>Ralstonia solanacearum</i> suppressed the development of wilt symptoms to less than one third of the control. This disease suppression in tomato plants was reproduced by pretreating their roots with a cell-free filtrate (CF) that had been fractionated from <i>B. thuringiensis</i> culture by centrifugation and filtration. In tomato plants challenge-inoculated with <i>R. solanacearum</i> after pretreatment with CF, the growth of <i>R. solanacearum</i> in stem tissues clearly decreased, and expression of defense-related genes such as PR-1, acidic chitinase, and <i>β</i>-1,3-glucanase was induced in stem and leaf tissues. Furthermore, the stem tissues of tomato plants with their roots were pretreated with CF exhibited resistance against direct inoculation with <i>R. solanacearum</i>. Taken together, these results suggest that treatment of tomato roots with the CF of <i>B. thuringiensis</i> systemically suppresses bacterial wilt through systemic activation of the plant defense system.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 28(1), 128-134, 2013-03-01

    Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology

References:  54

Codes

  • NII Article ID (NAID)
    10031138553
  • NII NACSIS-CAT ID (NCID)
    AA11173196
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13426311
  • Data Source
    CJP  J-STAGE 
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