Myocardial Layer-Specific Effect of Myoblast Cell-Sheet Implantation Evaluated by Tissue Strain Imaging

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Author(s)

    • SHUDO Yasuhiro
    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine
    • MIYAGAWA Shigeru
    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine
    • NAKATANI Satoshi
    • Department of Health Sciences, Division of Functional Diagnostics, Osaka University Graduate School of Medicine
    • FUKUSHIMA Satsuki
    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine
    • SAKAGUCHI Taichi
    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine
    • SAITO Atsuhiro
    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine
    • ASANUMA Toshihiko
    • Department of Health Sciences, Division of Functional Diagnostics, Osaka University Graduate School of Medicine
    • SHIMIZU Tatsuya
    • Advanced Biomedical Engineer and Science, Tokyo Women's Medical University
    • OKANO Teruo
    • Advanced Biomedical Engineer and Science, Tokyo Women's Medical University
    • SAWA Yoshiki
    • Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine

Abstract

<b><i>Background:</i></b> The implantation of skeletal myoblast (SMB) cell-sheets over the damaged area of a myocardial infarction (MI) has been shown to improve global left ventricular (LV) function through a paracrine effect. However, the regeneration process has not been fully evaluated. We hypothesized that the use of tissue Doppler strain M-mode imaging to assess myocardial layer-specific strain might enable detailed visual evaluation of the regenerative ability of SMBs. <b><i>Methods and Results:</i></b> SMBs were cultured on temperature-responsive culture dishes to generate cell-sheets. At 4 weeks after inducing anterior MI, the animals were divided into 2 groups: SMB cell-sheet implantation and sham operation (n=6 in each). A total of 30 cell-sheets (1.5×10<sup>7</sup> cells/sheet) were placed on the epicardium, covering the infarct and border regions. Subendocardial and subepicardial strain values were measured in the infarct, border, and remote regions by tissue Doppler strain analysis. SMB cell-sheet implantation produced the following major effects: progression of LV remodeling was prevented and global LV ejection fraction increased; the subendocardial strain was significantly greater than the subepicardial strain in the treated border region; vascular density in the subendocardium was significantly higher than in the subepicardium in the treated region; the expression of vascular endothelial growth factor was significantly increased. <b><i>Conclusions:</i></b> Tissue Doppler strain analysis allows precise evaluation of the effect of cell-sheet implantation on layer-specific myocardial function.  (Circ J 2013; 77: 1063–1072)<br>

Journal

  • Circulation Journal

    Circulation Journal 77(4), 1063-1072, 2013-03-25

    The Japanese Circulation Society

References:  39

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