ヒトゲノムDNAの不規則で柔軟な収納原理  [in Japanese] Irregular Folding of Human Genome DNA in the Cells  [in Japanese]

Access this Article

Author(s)

Abstract

How is 2-m-long genomic DNA organized into a mitotic chromosome or nucleus? The nucleosome fiber has long been assumed to be folded into a 30-nm chromatin fiber, and further helically folded larger fiber. However, when we observed frozen hydrated human mitotic cells using cryoelectron microscopy (cryo-EM), no higher-order structures including 30-nm chromatin fibers were found. To further investigate the bulk structure of mitotic chromosomes, we performed small-angle X-ray scattering (SAXS) at SPring-8. No structural feature larger than 11 nm was detected, even on a chromosome-diameter scale (~1 μm). We also found a similar scattering pattern in interphase nuclei of HeLa cells in the range up to ~275 nm. Our findings suggest a common structural feature in interphase and mitotic chromatins: compact and irregular folding of nucleosome fibers occurs without a 30-nm chromatin structure.<br>

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 53(1), 4-10, 2013-01-25

    The Biophysical Society of Japan General Incorporated Association

References:  29

  • <no title>

    ELTSOV M.

    Proc. Natl. Acad. Sci. USA 105, 19732-19737, 2008

    Cited by (1)

  • <no title>

    NIAHINO Y.

    EMBO J. 31, 1644-1653, 2012

    Cited by (1)

  • <no title>

    JOTI Y.

    Nucleus 3, 404-410, 2012

    Cited by (1)

  • <no title>

    KORNBERG R. D.

    Cell 98, 285-294, 1999

    Cited by (1)

  • <no title>

    FINCH J. T.

    Proc. Natl. Acad. Sci. USA 73, 1897-1901, 1976

    Cited by (1)

  • <no title>

    SEDAT J.

    Cold Spring Harb. Symp. Quant. Biol. 42 Pt 1, 331-350, 1978

    Cited by (1)

  • <no title>

    DUBOCHET J.

    Micron 32, 91-99, 2001

    Cited by (1)

  • <no title>

    MAESHIMA K.

    Cold Spring Harb. Symp. Quant. Biol. 75, 439-444, 2010

    Cited by (1)

  • <no title>

    MAESHIMA K.

    J. Biochem. (Tokyo) 143, 145-153, 2008

    Cited by (1)

  • <no title>

    MCDOWALL A. W.

    EMBO J 5, 1395-1402, 1986

    Cited by (1)

  • <no title>

    FRANK J.

    Three-Dimensional Electron Microscopy of Macromolecular Assembly, 2006

    Cited by (1)

  • <no title>

    ROE R.-J.

    Methods of X-Ray and Neutron Scattering in Polymer Science, 2000

    Cited by (1)

  • <no title>

    LANGMORE J. P.

    J. Cell Biol. 96, 1120-1131, 1983

    Cited by (1)

  • <no title>

    PAULSON J. R.

    J. Cell Biol. 96, 1132-1137, 1983

    Cited by (1)

  • <no title>

    NISHINO Y.

    Physical Review Letters 102, 18101, 2009

    Cited by (1)

  • <no title>

    HANSEN J. C.

    EMBO J. 31, 1621-1623, 2012

    Cited by (1)

  • <no title>

    BOUCHET-MARQUIS C.

    Histochem. Cell Biol. 125, 43-51, 2006

    Cited by (1)

  • <no title>

    FAKAN S.

    Semin Cell Dev. Biol. 18, 676-681, 2007

    Cited by (1)

  • <no title>

    FUSSNER E.

    Trends Biochem. Sci. 36, 1-6, 2011

    Cited by (1)

  • <no title>

    FUSSNER E.

    EMBO J. 30, 1778-1789, 2011

    Cited by (1)

  • <no title>

    LIEBERMAN-AIDEN E.

    Science 326, 289-293, 2009

    Cited by (1)

  • <no title>

    MAESHIMA K.

    Curr. Opin Cell Biol. 22, 291-297, 2010

    Cited by (1)

  • <no title>

    YOSHIKAWA K.

    Compaction and Condensation of DNA, 137-163, 2002

    Cited by (1)

  • <no title>

    MAESHIMA K.

    Chromosoma 114, 365-375, 2005

    Cited by (1)

  • <no title>

    SCHEFFER M. P.

    J. Struct. Biol. 178, 207-214, 2012

    Cited by (1)

  • <no title>

    KIZILYAPRAK C.

    PLoS One 5, e11039, 2010

    Cited by (1)

  • <no title>

    TAVORMINA P. A.

    J. Cell Biol. 158, 23-29, 2002

    Cited by (1)

  • <no title>

    GERLICH D.

    Curr. Biol. 16, 333-344, 2006

    Cited by (1)

  • <no title>

    HIHARA S.

    Cell Rep. 2, 1645-1656, 2012

    Cited by (1)

Codes

  • NII Article ID (NAID)
    10031139018
  • NII NACSIS-CAT ID (NCID)
    AN00129693
  • Text Lang
    JPN
  • Article Type
    REV
  • ISSN
    05824052
  • NDL Article ID
    024274438
  • NDL Call No.
    Z18-49
  • Data Source
    CJP  NDL  J-STAGE 
Page Top