光子・電子ハイブリッド顕微鏡開発 : 「FL-SEM」による観察  [in Japanese] A Fluorescence Scanning Electron Microscope  [in Japanese]

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Author(s)

Abstract

<p>生体の機能と形態の関連を裏付ける可能性を秘めた蛍光組織細胞化学染色標本において,同時・同軸での超微形態観察が実現できれば,微小領域の形態と機能の統合的解析が可能となる.今回,蛍光光学顕微鏡と走査型電子顕微鏡を組み合わせたハイブリッド型顕微鏡を開発した.本稿では「FL-SEM」と命名した本装置の概要を解説し,撮影された画像を紹介する.また本装置による観察に有利な新規蛍光物質「Fluolid」について言及する.</p>

<p>Devices that enable simultaneous and the same axis observation of histocytochemically labeled ultrastructure by fluorescence probes are desired for more detailed biomedical experiments and understanding of the functional molecular morphology. We have developed a hybrid microscope equipped with both fluorescent optical microscope and scanning electron microscope. In this article, we will briefly review the trial processes of the FL-SEM development, and show some examples obtained by the instrument. Fluolid, a novel fluorescent substance that is favorable for utilizing in the hybrid microscope, is also mentioned.</p>

Journal

  • KENBIKYO

    KENBIKYO 46(1), 66-70, 2011-03-30

    The Japanese Society of Microscopy

References:  14

Cited by:  1

  • Present and Future Views in SEM Techniques  [in Japanese]

    INAZATO Sachiko , OTOBE Hirohide , YAMASHITA Mika , YONEMITSU Kyouko , WATANABE Syunya , KONOMI Mami , KUBA Toshiko , SHIBATA Masateru , MORIYA Hisao , SATO Mamiko , SHINDO Emi , MURAI Noriko

    顕微鏡 = Microscopy 48(2), 133-141, 2013-08-30

    References (14)

Codes

  • NII Article ID (NAID)
    10031144731
  • NII NACSIS-CAT ID (NCID)
    AA11917781
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    13490958
  • NDL Article ID
    11083637
  • NDL Source Classification
    ZN33(科学技術--電気工学・電気機械工業--電子工学・電気通信)
  • NDL Call No.
    Z16-896
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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