Functional Analysis of Degradative Enzymes for Hydroxyproline-linked β-L-Arabinofuranosides in <i>Bifidobacterium longum</i>

• Fujita Kiyotaka

  Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan

• Kitahara Kanefumi

  Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan

• Suganuma Toshihiko

  Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan

Bibliographic Information

Other Title

• <i>Bifidobacterium longum</i> が生産するβ- アラビノオリゴ糖鎖分解酵素群の機能解析
• Functional Analysis of Degradative Enzymes for Hydroxyproline-linked β-L-Arabinofuranosides in Bifidobacterium longum
• Functional Analysis of Degradative Enzymes for Hydroxyproline-linked ^|^beta;-L-Arabinofuranosides in Bifidobacterium longum
• Functional analysis of degradative enzymes for hydroxyproline-linked -L-arabinofuranosides in Bifidobacterium longum

Abstract

β-L-Arabinofuranosides are hydroxyproline (Hyp)-linked sugar chains of extensin observed in plant cell wall fractions. Despite the broad distribution of β-L-arabinofuranosyl residues in plants, degradative enzymes have not yet been identified. In 2011, we cloned and characterized the first degradative enzymes for Hyp-linked β-L-arabinofuranosides from Bifidobacterium longum. These enzymes were composed of a glycoside hydrolase (GH) family 43 α-L-arabinofuranosidase (HypAA) releasing L-arabinose from Arafα1-3Arafβ1-2Arafβ1-2Arafβ-Hyp, a GH121 β-L-arabinobiosidase (HypBA2) releasing Arafβ1-2Araf (β-Ara2) from Arafβ1-2Arafβ1-2Arafβ-Hyp, and a GH127 β-L-arabinofuranosidase (HypBA1) degrading β-Ara2 to L-arabinose. These enzymes are encoded in a conserved gene cluster on several B. longum genomes, but not in genome sequences of other intestinal bacteria. Hyp-linked β-L-arabinofuranosides were utilized as carbohydrate sources by B. longum. This review presents the functional features of enzymes for Hyp-linked β-L-arabinofuranosides and the predicted metabolic pathway in B. longum.

Journal

• Trends in Glycoscience and Glycotechnology

  Trends in Glycoscience and Glycotechnology 24 (139), 215-224, 2012

  FCCA(Forum: Carbohydrates Coming of Age)

Keywords

• extensin
• hydroxyproline-rich glycoprotein
• β-L-arabinofuranosides
• <i>Bifidobacterium longum</i>
• arabinofuranosidase
• prebiotics

Details

• CRID: 1390282679346768128
• NII Article ID: 10031146673
• NII Book ID: AA10995236
• DOI: 10.4052/tigg.24.215
• COI: 1:CAS:528:DC%2BC3sXit1Gls7w%3D
• ISSN: 18832113
• ISSN: 09157352
• NDL BIB ID: 024179898
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024179898
• Web Site: https://search.jamas.or.jp/link/ui/2013148935
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed