Proteomic Analysis of the <i>Streptomyces griseus</i> Ribosomal Fraction

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  • AKANUMA Genki
    Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
  • NANAMIYA Hideaki
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • MOURI Yoshihiro
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • ISHIZUKA Morio
    Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
  • OHNISHI Yasuo
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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  • Proteomic Analysis of the Streptomyces griseus Ribosomal Fraction

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Abstract

The Streptomyces griseus 70S ribosome fraction was analyzed by radical-free and highly reducing two-dimensional (RFHR 2D) gel electrophoresis and mass spectrometry. Among the 60 putative ribosomal proteins that are encoded by the S. griseus genome, 48 were identified in the 70S ribosome fraction prepared from mycelia grown in liquid culture for 12, 36, and 48 h. Ribosomal protein S3 was detected at two different positions on the 2D gel, and the distribution changed completely in the course of the growth, suggesting that it was modified or processed. The SGR3624 protein was also identified in the 70S ribosome fraction, but detailed cellular fractionation analysis indicated that it localizes mainly at the membrane rather than the ribosome. An SGR3624-deleted mutant showed slow growth on solid media, indicating that SGR3624 has an important role in the growth of the substrate mycelium in solid culture.

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