Indirect UV Detection–Ion-exclusion/Cation-exchange Chromatography of Common Inorganic Ions with Sulfosalicylic Acid Eluent

• KOZAKI Daisuke

  Japan Society for the Promotion of Science Graduate School of Engineering, Gunma University

• MORI Masanobu

  Graduate School of Engineering, Gunma University

• NAKATANI Nobutake

  Department of Environmental and Symbiotic Sciences, College of Agriculture, Food and Environmental Science, Rakuno Gakuen University

• ARAI Kaori

  Graduate School of Engineering, Gunma University

• MASUNO Tomoe

  Graduate School of Engineering, Gunma University

• KOSEKI Masakazu

  Graduate School of Engineering, Gunma University

• ITABASHI Hideyuki

  Graduate School of Engineering, Gunma University

• TANAKA Kazuhiko

  Graduate School for International Development and Cooperation, Hiroshima University

Abstract

Herein, we describe indirect UV detection–ion-exclusion/cation-exchange chromatography (IEC/CEC) on a weakly acidic cation-exchange resin in the H⁺-form (TSKgel Super IC-A/C) using sulfosalicylic acid as the eluent. The goal of the study was to characterize the peaks detected by UV detector. The peak directions of analyte ions in UV at 315 nm were negative because the molar absorbance coefficients of analyte anions and cations were lower than that of the sulfosalicylic acid eluent. Good chromatographic resolution and high signal-to-noise ratios of analyte ions were obtained for the separations performed using 1.1 mM sulfosalicylic acid and 1.5 mM 18-crown-6 as the eluent. The relative standard deviations (RSDs) of the peak areas ranged from 0.6 to 4.9%. Lower detection limits of the analytes were achieved using indirect UV detection at 315 nm (0.23 – 0.98 μM) than those obtained with conductometric detection (CD) (0.61 – 2.1 μM) under the optimized elution conditions. The calibration curves were linear in the range from 0.01 to 1.0 mM except for Cl⁻, which was from 0.02 to 2.0 mM. The present method was successfully applied to determine common inorganic ions in a pond water sample.

Journal

• Analytical Sciences

  Analytical Sciences 29 (1), 121-126, 2013

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390282679235832320
• NII Article ID: 10031147337
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.29.121
• COI: 1:STN:280:DC%2BC3s3otFKlsA%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 024212207
• PubMed: 23303096
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024212207
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.29.121.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.29.121/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/29/1/29_121/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed