Effect of a Low-conductivity Zone on Field-amplified Sample Stacking in Microchip Micellar Electrokinetic Chromatography
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- SUEYOSHI Kenji
- Department of Material Chemistry, Graduate School of Engineering, Kyoto University
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- KITAGAWA Fumihiko
- Department of Material Chemistry, Graduate School of Engineering, Kyoto University
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- OTSUKA Koji
- Department of Material Chemistry, Graduate School of Engineering, Kyoto University
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The effects of a low-conductivity (low-C) zone of an on-line sample preconcentration based on field-amplified sample stacking (FASS) under an acidic condition was investigated in microchip micellar electrokinetic chromatography (MCMEKC). By employing originally fabricated microchips with T- and cross-channel injectors on a single device, partial injection of the low-C zone and large-volume injection of a sample solution were demonstrated. As a typical result, the observed peak intensities of the fluorescent dyes were 1.5 – 2.0-fold enhanced by partial injection of the low-conductivity zone, as compared to those in FASS-MCMEKC without injecting the low-C zone; also, 30 – 40-fold enhancements of the detectability were achieved as compared to the conventional MCMEKC analyses. The resolutions of the hydrophobic analytes were also improved by introducing the low-C zone. The difference in the retention factors of model analytes in the high-C background and low-C zones was also estimated in order to clarify the effect of the low-C zone on the resolution and detectability in FASS-MCMEKC.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 29 (1), 133-138, 2013
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679235800192
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- NII論文ID
- 10031147339
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC3s3otFKltg%3D%3D
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- ISSN
- 13482246
- 09106340
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- HANDLE
- 2433/193726
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- NDL書誌ID
- 024212234
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- PubMed
- 23303098
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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