Fine mapping of the clubroot resistance gene CRb and development of a useful selectable marker in Brassica rapa

• Kato Takeyuki

  Graduate School of Bioresources, Mie University NARO Institute of Vegetable and Tea Science

• Hatakeyama Katsunori

  NARO Institute of Vegetable and Tea Science

• Fukino Nobuko

  NARO Institute of Vegetable and Tea Science

• Matsumoto Satoru

  NARO Institute of Vegetable and Tea Science

書誌事項

タイトル別名

• Fine mapping of the clubroot resistance gene <i>CRb</i> and development of a useful selectable marker in <i>Brassica rapa</i>

抄録

In Chinese cabbage (Brassica rapa), the clubroot resistance (CR) gene CRb is effective against Plasmodiophora brassicae isolate No. 14, which is classified as pathotype group 3. Although markers linked to CRb have been reported, an accurate position in the genome and the gene structure are unknown. To determine the genomic location and estimate the structure of CRb, we developed 28 markers (average distance, 20.4 kb) around CRb and constructed a high-density partial map. The precise position of CRb was determined by using a population of 2,032 F₂ plants generated by selfing B. rapa ‘CR Shinki.’ We determined that CRb is located in the 140-kb genomic region between markers KB59N07 and B1005 and found candidate resistance genes. Among other CR genes on chromosome R3, a genotype of CRa closest marker clearly matched those of CRb and Crr3 did not confer resistance to isolate No. 14. Based on the genotypes of 11 markers developed near CRb and resistance to isolate No. 14, 82 of 108 cultivars showed a strong correlation between genotypes and phenotypes. The results of this study will be useful for isolating CRb and breeding cultivars with resistance to pathotype group 3 by introducing CRb into susceptible cultivars through marker-assisted selection.

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 63 (1), 116-124, 2013

  日本育種学会

キーワード

• clubroot
• <i>Plasmodiophora brassicae</i>
• <i>CRb</i>
• fine mapping
• marker-assisted selection
• <i>Brassica rapa</i>

詳細情報

• CRID: 1390282679700209664
• NII論文ID: 10031151531
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.63.116
• COI: 1:CAS:528:DC%2BC3sXptVOrsbc%3D
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 024360055
• PubMed: 23641188
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024360055
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/63/1/63_116/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可