Ultrastructural study on blood vessels and lymphatic vessels invaded by rabbit VX2 tongue cancer

• Furihata Kyuji

  First Department of Oral and Maxillofacial Surgery, School of Dentistry, Iwate Medical University

Bibliographic Information

Other Title

• ウサギ VX2 舌癌の血管とリンパ管への侵襲に関する超微細構造の観察

Abstract

Mode of invasion into blood vessels and lymphatic vessels of VX2 tongue cancer was morphologically investigated in order to clarify a metastatic mechanism of the cancer to cervical lymph nodes. The suspension of VX2 cancer cells was transplanted into the rabbit tongue muscle. The tongue was excised on the 7 th and l4th days after transplantation. The excised specimen was processed for light and transmission electron microscopy. On the light microscopic examination the peripheral portions of VX2 cancer nests revealed irregular boundaries, and the cancer cells invaded the vascular vessels at these portions. Observation with the transmission electron microscopy showed invasion of cancer cells into the venules and lymphatic vessels. Immunocytes were more frequently observed in the venules than in the lymphatic vessels. The invading cancer cells had no basernent membranes and connected each other loosely. Between the endothelial cells of the both venule/lymphatic vessel and the cancer cells, focal contacts were seen as high density electron bands. The interendothelial space of each vascular endothelium was open in the areas invaded by the cancer cells. The three-dimensionally reconstructed image showed that the cancer cells have invaded into the internal lumen of the lymphatic vessel at only one place. The results of this study suggest that VX2 cancer cells existing in the peripheral portion of the cancer nest has invaded into lumen through the interendothelial space of both venule and lymphatic endotheliums.

Journal

• Dental Journal of Iwate Medical University

  Dental Journal of Iwate Medical University 24 (3), 194-205, 1999

  The Dental Society of Iwate Medical University

Keywords

• vascular invasion
• VX2 tongue cancer
• interendothelial space
• focal contact

Details

• CRID: 1390282679568430464
• NII Article ID: 10031152392
• NII Article ID: 130006837811
• NII Article ID: 80010799403
• NII Book ID: AN00017474
• DOI: 10.20663/iwateshigakukaishi.24.3_194
• ISSN: 24241822
• ISSN: 03851311
• Web Site: http://id.nii.ac.jp/1181/00002855/
• Web Site: https://search.jamas.or.jp/link/ui/2000177353
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • CiNii Articles
• Abstract License Flag: Disallowed