A Pilot Study for the Mutation Assay Using a High-throughput DNA Sequencer

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Author(s)

Abstract

We present here a mutation assay with little bias which incorporates high-throughput DNA sequencing technology. Our strategy is simple: 1) expose cells to a test compound, 2) isolate colonies, and 3) carry out whole-genome sequencing of the clones. In this pilot study, we used <i>Salmonella typhimurium</i> TA100 as a tester strain and successfully detected mutations induced by the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2). We believe that this new mutation assay will be a very useful tool in hazard assessment of chemicals.<br>

Journal

  • Genes and Environment

    Genes and Environment 35(2), 53-56, 2013-05-20

    The Japanese Environmental Mutagen Society

Codes

  • NII Article ID (NAID)
    10031158581
  • NII NACSIS-CAT ID (NCID)
    AA1212552X
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    18807046
  • NDL Article ID
    024474905
  • NDL Call No.
    Z78-A397
  • Data Source
    CJP  NDL  J-STAGE 
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