Differential Resuscitative Effect of Pyruvate and its Analogues on VBNC (Viable But Non-Culturable) <i>Salmonella</i>

• Morishige Yuta

  Laboratory of Biodefense & Regulation, Osaka University of Pharmaceutical Sciences

• Fujimori Ko

  Laboratory of Biodefense & Regulation, Osaka University of Pharmaceutical Sciences

• Amano Fumio

  Laboratory of Biodefense & Regulation, Osaka University of Pharmaceutical Sciences

Bibliographic Information

Other Title

• Differential Resuscitative Effect of Pyruvate and its Analogues on VBNC (Viable But Non-Culturable) Salmonella

Abstract

An environmental isolate of Salmonella Enteritidis (SE), grown to the logarithmic phase, rapidly lost culturability by the addition of 3 mM H₂O₂ to cultures grown in Luria-Bertani (LB) medium; however, some H₂O₂-treated bacteria regained their culturability in M9 minimal medium, if sodium pyruvate was present at at least 0.3 mM. In addition, most pyruvate analogues, such as bromopyruvate or phenylpyruvate, did not show restoration activity similar to that of pyruvate, except in the case of α-ketobutyrate. Further analysis of the mechanism underlying the resuscitation by pyruvate revealed that although many of the bacteria showed respiratory activity on CTC (5-cyano-2,3-di-(p-tolyl) tetrazolium chloride) reduction with or without pyruvate, the biosynthesis of DNA and protein synthesis were quite different in the presence or absence of pyruvate, i.e., pyruvate endowed the cells with the ability to incorporate much more radio-label into precursors during the resuscitation process. These results suggest that pyruvate is one of the key molecules working in the resuscitation process by taking bacteria from the non-culturable state to the growing and colony-forming state by triggering the synthesis of macromolecules such as DNA and protein.<br>

Journal

• Microbes and Environments

  Microbes and Environments 28 (2), 180-186, 2013

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• VBNC (viable but non-culturable)
• <i>Salmonella</i>
• pyruvate
• resuscitation
• H₂O₂

Details

• CRID: 1390282679322492288
• NII Article ID: 10031158620
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me12174
• COI: 1:STN:280:DC%2BC3srmtVKrug%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 024530129
• PubMed: 23595023
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024530129
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/28/2/28_ME12174/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed