Candidatus Methanogranum caenicola : a Novel Methanogen from the Anaerobic Digested Sludge, and Proposal of Methanomassiliicoccaceae fam. nov. and Methanomassiliicoccales ord. nov., for a Methanogenic Lineage of the Class Thermoplasmata

• Iino Takao

  Japan Collection of Microorganisms, RIKEN BioResource Center

• Tamaki Hideyuki

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

• Tamazawa Satoshi

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Graduate School of Life and Environmental Sciences, University of Tsukuba

• Ueno Yoshiyuki

  Kajima Technical Research Institute

• Ohkuma Moriya

  Japan Collection of Microorganisms, RIKEN BioResource Center

• Suzuki Ken-ichiro

  NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE)

• Igarashi Yasuo

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Haruta Shin

  Graduate School of Science and Engineering, Tokyo Metropolitan University

書誌事項

タイトル別名

• <i>Candidatus</i> Methanogranum caenicola: a Novel Methanogen from the Anaerobic Digested Sludge, and Proposal of <i>Methanomassiliicoccaceae</i> fam. nov. and <i>Methanomassiliicoccales</i> ord. nov., for a Methanogenic Lineage of the Class <i>Thermoplasmata</i>

抄録

The class Thermoplasmata harbors huge uncultured archaeal lineages at the order level, so-called Groups E2 and E3. A novel archaeon Kjm51a affiliated with Group E2 was enriched from anaerobic sludge in the present study. Clone library analysis of the archaeal 16S rRNA and mcrA genes confirmed a unique archaeal population in the enrichment culture. The 16S rRNA gene-based phylogeny revealed that the enriched archaeon Kjm51a formed a distinct cluster within Group E2 in the class Thermoplasmata together with Methanomassiliicoccus luminyensis B10^T and environmental clone sequences derived from anaerobic digesters, bovine rumen, and landfill leachate. Archaeon Kjm51a showed 87.7% 16S rRNA gene sequence identity to the closest cultured species, M. luminyensis B10^T, indicating that archaeon Kjm51a might be phylogenetically novel at least at the genus level. In fluorescence in situ hybridization analysis, archaeon Kjm51a was observed as coccoid cells completely corresponding to the archaeal cells detected, although bacterial rod cells still coexisted. The growth of archaeon Kjm51a was dependent on the presence of methanol and yeast extract, and hydrogen and methane were produced in the enrichment culture. The addition of 2-bromo ethanesulfonate to the enrichment culture completely inhibited methane production and increased hydrogen concentration, which suggested that archaeon Kjm51a is a methanol-reducing hydrogenotrophic methanogen. Taken together, we propose the provisional taxonomic assignment, named Candidatus Methanogranum caenicola, for the enriched archaeon Kjm51a belonging to Group E2. We also propose to place the methanogenic lineage of the class Thermoplasmata in a novel order, Methanomassiliicoccales ord. nov.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 28 (2), 244-250, 2013

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• Methanogranum caenicola
• methanogen
• <i>Thermoplasmata</i>
• rice cluster III
• anaerobic digested sludge

詳細情報

• CRID: 1390282679322484864
• NII論文ID: 10031158628
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me12189
• COI: 1:STN:280:DC%2BC3svptVSltA%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 024530261
• PubMed: 23524372
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024530261
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/28/2/28_ME12189/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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