Angiopoietin-1 Mediates Adipose Tissue-Derived Stem Cell-Induced Inhibition of Neointimal Formation in Rat Femoral Artery

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Author(s)

    • TAKAHASHI Masao
    • Department of Internal Medicine, Faculty of Medicine, University of Tokyo
    • SUZUKI Etsu
    • Institute of Medical Science, St. Marianna University School of Medicine
    • OBA Shigeyoshi
    • Department of Internal Medicine, Faculty of Medicine, University of Tokyo
    • SATO Tomohiko
    • Department of Internal Medicine, Faculty of Medicine, University of Tokyo
    • KIMURA Kenjiro
    • Division of Nephrology and Hypertension, St. Marianna University School of Medicine
    • NAGANO Tetsuo
    • Graduate School of Pharmaceutical Sciences, University of Tokyo
    • HIRATA Yasunobu
    • Department of Internal Medicine, Faculty of Medicine, University of Tokyo

Abstract

<b><i>Background:</i></b> Adipose tissue-derived stem cells (ASC) produce a variety of cytokines that potentially mediate the proangiogenic and antiapoptotic effects of the ASC. We examined whether ASC produced angiopoietin-1 (Ang1) and whether Ang1 functionally mediated ASC-induced suppression of neointimal formation. <b><i>Methods and Results:</i></b> Ang1 production was measured by enzyme-linked immunosorbent assay. Production of endogenous Ang1 by ASC was inhibited with small interfering RNA (siRNA) for Ang1. Overproduction of Ang1 was achieved with an adenovirus that expresses Ang1 (AdAng1). ASC expressing Ang1 siRNA, or AdAng1 were administered around the femoral artery after wire injury, and immunohistochemical analysis was performed to examine their effects on neointimal formation. ASC produced Ang1 in a time-dependent manner, especially when cultured in medium containing growth factors for vascular endothelial cells. When ASC were treated with Ang1 siRNA, the inhibitory effect of ASC on neointimal formation was significantly reduced. Knockdown of Ang1 significantly increased macrophage infiltration in the neointima, and significantly decreased endothelial regeneration. In contrast, forced expression of Ang1 using AdAng1 significantly suppressed neointimal formation and macrophage infiltration, and stimulated reendothelialization. <b><i>Conclusions:</i></b> Ang1 was implicated in ASC-induced suppression of neointimal formation. The results also suggested that Ang1 inhibited neointimal formation via stimulation of reendothelialization and suppression of macrophage infiltration in the neointima.  (Circ J 2013; 77: 1574–1584)<br>

Journal

  • Circulation Journal

    Circulation Journal 77(6), 1574-1584, 2013-05-25

    The Japanese Circulation Society

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