Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells

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Author(s)

Abstract

We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours’ treatment with the particles dose-dependently increased the frequency of micronuclei (MN) in the A549 and CHO AA8 cells up to 5.2% and 5.0% at a dose of 200 µg/ml (34 µg/cm<sup>2</sup>), respectively. In A549 cells, treatment with the nano­particles (2 µg/ml) for 1 hr induced H2AX phosphorylation, which is suggestive of DNA double strand breaks (DSB). Treating CHO AA8 cells with 2 µg/ml (0.34 µg/cm<sup>2</sup>) magnetite for 1 hour resulted in a five times higher frequency of sister chromatid exchange (SCE) than the control level. We detected reactive oxygen species (ROS) in CHO cells treated with the particles. These findings indicate that magnetite nano­particles induce ROS in mammalian cells, leading to the direct or indirect induction of DSB, followed by clastogenic events including MN and SCE.

Journal

  • The Journal of Toxicological Sciences

    The Journal of Toxicological Sciences 38(3), 503-511, 2013-06-01

    The Japanese Society of Toxicology

References:  26

Codes

  • NII Article ID (NAID)
    10031158916
  • NII NACSIS-CAT ID (NCID)
    AN00002808
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    03881350
  • NDL Article ID
    024784154
  • NDL Call No.
    Z19-1022
  • DOI
    10.2131/jts.38.503
  • Data Source
    CJP  NDL  J-STAGE 
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