Exploring Sequences for Thermally Stable Proteins by the Ancestral Design Method

• AKANUMA Satoshi

  Department of Applied Life Sciences, Tokyo University of Pharmacy and Life Sciences

Bibliographic Information

Other Title

• 祖先型設計法を用いた耐熱性タンパク質の配列探索
• ソセンガタ セッケイホウ オ モチイタ タイネツセイ タンパクシツ ノ ハイレツ タンサク

Abstract

We developed the ancestral design method that incorporates a multiple amino acid sequence alignment of homologous proteins and construction of the related phylogenetic tree to infer the ancestral sequence. Ancestral residues were introduced into several thermophilic proteins, producing mutant proteins with further enhanced thermostability. We also predicted, synthesized, and characterized the complete ancestral sequences of B subunit of DNA gyrase. The ancestral protein showed thermostability similar to a modern thermophilic protein. Thus, the ancestral design will serve as an effective method for creating thermally stable proteins that only relies on the amino acid sequences of homologous proteins.<br>

Journal

• Seibutsu Butsuri

  Seibutsu Butsuri 53 (3), 128-133, 2013

  The Biophysical Society of Japan General Incorporated Association

Keywords

• ancestral design
• consensus design
• homologous protein sequences
• multiple sequence alignment
• phylogenetic tree
• thermally stable protein

Details

• CRID: 1390282681511838976
• NII Article ID: 10031159063
• NII Book ID: AN00129693
• DOI: 10.2142/biophys.53.128
• COI: 1:CAS:528:DC%2BC3sXptFSisr8%3D
• ISSN: 13474219
• ISSN: 05824052
• NDL BIB ID: 024755507
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024755507
• Web Site: https://www.jstage.jst.go.jp/article/biophys/53/3/53_128/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed