塩蔵ダイコンの抗変異原性物質の同定と定量解析  [in Japanese] Identification and Quantification of Antimutagenic Compounds in Salted Radish  [in Japanese]

Access this Article

Search this Article

Author(s)

Abstract

Ames試験により塩蔵ダイコンのアセトニトリル抽出物中に抗変異原性を有する画分を見いだした.機器分析により,活性成分の一つとしてパルミチン酸を同定した.そこで,塩蔵ダイコンおよび市販タクアン漬け製品について,脂肪酸の定量解析を行った.主要な脂肪酸として,α-リノレン酸,リノール酸およびパルミチン酸が見いだされた.その他にパルミトレイン酸,ステアリン酸,オレイン酸および(<I>Z</I>)-バクセン酸などが存在した.干しタクアン漬試料の総脂肪酸量は274-680 μmol/100 g,塩押しダイコン由来の試料では124∼357 μmol/100 gであった.また,どちらの試料も脱水処理や塩蔵熟成中に遊離脂肪酸の比率が相対的に高くなった.一方,干したくあん漬けの脂肪酸組成比は新鮮ダイコンとほぼ同等であったが,塩押しダイコン由来の試料では多価不飽和脂肪酸の比率が相対的に低くなった.さらに,塩蔵ダイコンの主要な脂肪酸について,Ames および<I>umu</I>試験を用いて抗変異原性試験を行った.Ames試験において,(<I>Z</I>)-バクセン酸の 4NQO に対する抑制活性は他の脂肪酸よりも高く,ED<SUB>50</SUB>値は 2.8 nmol/plate であった.

We identified an antimutagenic fraction in acetonitrile extracts from salted radish by Ames assay. One of the active compounds was identified as palmitic acid by NMR and GC-MS. Quantitative analysis of fatty acids was performed in salted radishes and commercial <I>takuan-zuke</I>. The major fatty acids were identified as α-linolenic, linoleic and palmitic acid, while the minor fatty acids were identified as palmitoleic, stearic, linoleic, oleic and (<I>Z</I>)-vaccenic acid. Total acyls in dried <I>takuan-zuke </I>were 274-680 μmol/100 g, and total acyls in salted radish were 124-357 μmol/100 g. During dehydration and salted aging, the ratio of free fatty acids to total acyls increased. While the fatty acid composition of dried radish products was equivalent to that of fresh radish, the ratio of polyunsaturated fatty acids of salted radish products was relatively low. The major fatty acids from salted radish showed antimutagenic activity against 2-amino-3-methylimidazo[4,5-<I>ƒ</I>]quinoline (IQ) and 4-nitroquinoline 1-oxide (4NQO) on Ames and <I>umu </I>tests. The results for inhibitory activity against IQ on the <I>umu </I>test were more sensitive than on the Ames test. The antimutagenicity of (<I>Z</I>)-vaccenic acid was greater than that of other fatty acids in the 4NQO-Ames test system, and the ED 50 (50% effective dose) value was estimated to be 2.8 nmol/plate.

Journal

  • Nippon Shokuhin Kagaku Kogaku Kaishi

    Nippon Shokuhin Kagaku Kogaku Kaishi 60(3), 117-124, 2013-03-15

    Japanese Society for Food Science and Technology

References:  37

Codes

  • NII Article ID (NAID)
    10031163921
  • NII NACSIS-CAT ID (NCID)
    AN10467499
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    1341027X
  • NDL Article ID
    024336153
  • NDL Call No.
    Z17-126
  • Data Source
    CJP  NDL  J-STAGE  JASI 
Page Top