Visualization of the Mycelia of Wood-Rotting Fungi by Fluorescence <i>in Situ</i> Hybridization Using a Peptide Nucleic Acid Probe

• NAKADA Yuji

  Faculty of Agriculture, Tokyo University of Agriculture and Technology

• NAKABA Satoshi

  Faculty of Agriculture, Tokyo University of Agriculture and Technology

• MATSUNAGA Hiroshi

  Department of Wood Improvement, Forestry and Forest Products Research Institute

• FUNADA Ryo

  Faculty of Agriculture, Tokyo University of Agriculture and Technology

• YOSHIDA Makoto

  Faculty of Agriculture, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title

• Visualization of the Mycelia of Wood-Rotting Fungi by Fluorescence in Situ Hybridization Using a Peptide Nucleic Acid Probe

Abstract

White rot fungus, Phanerochaete chrysosporium, and brown rot fungus, Postia placenta, grown on agar plates, were visualized by fluorescence in situ hybridization (FISH) using a peptide nucleic acid (PNA) probe. Mycelia grown on wood chips were also clearly detected by PNA-FISH following blocking treatment. To the best of our knowledge, this is the first report on the visualization of fungi in wood by FISH.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 77 (2), 405-408, 2013

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• wood-rotting fungi
• <i>Phanerochaete chrysosporium</i>
• <i>Postia placenta</i>
• peptide nucleic acid
• fluorescence <i>in situ</i> hybridization

Details

• CRID: 1390282681456813440
• NII Article ID: 10031164752
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.120777
• COI: 1:STN:280:DC%2BC3szmsFKrtA%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 024296048
• PubMed: 23391931
• Web Site: http://id.ndl.go.jp/bib/024296048
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024296048
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed