Gene Cloning and Characterization of L-Ribulose 3-epimerase from Mesorhizobium loti and Its Application to Rare Sugar Production

• UECHI Keiko

  Rare Sugar Research Center, Kagawa University

• TAKATA Goro

  Rare Sugar Research Center, Kagawa University

• FUKAI Yoshinori

  Rare Sugar Research Center, Kagawa University

• YOSHIHARA Akihide

  Rare Sugar Research Center, Kagawa University

• MORIMOTO Kenji

  Rare Sugar Research Center, Kagawa University

書誌事項

タイトル別名

• Gene Cloning and Characterization of <small>L</small>-Ribulose 3-epimerase from <i>Mesorhizobium loti</i> and Its Application to Rare Sugar Production
• Gene Cloning and Characterization of<scp>L</scp>-Ribulose 3-epimerase from<i>Mesorhizobium loti</i>and Its Application to Rare Sugar Production

抄録

A gene encoding L-ribulose 3-epimerase (L-RE) from Mesorhizobium loti, an important enzyme for rare sugar production by the Izumoring strategy, was cloned and overexpressed. The enzyme showed highest activity toward L-ribulose (230 U/mg) among keto-pentoses and keto-hexoses. This is the first report on a ketose 3-epimerase showing highest activity toward keto-pentose. The optimum enzyme reaction conditions for L-RE were determined to be sodium phosphate buffer (pH 8.0) at 60 °C. The enzyme showed of higher maximum reaction a rate (416 U/mg) and catalytic efficiency (43 M⁻¹ min⁻¹) for L-ribulose than other known ketose 3-epimerases. It was able to produce L-xylulose efficiently from ribitol in two-step reactions. In the end, 7.2 g of L-xylulose was obtained from 20 g of ribitol via L-ribulose at a yield of 36%.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (3), 511-515, 2013

  公益社団法人 日本農芸化学会

キーワード

• rare sugar
• <small>L</small>-ribulose 3-epimerase
• <small>L</small>-ribulose
• <small>L</small>-xylulose
• <i>Mesorhizobium loti</i>

詳細情報

• CRID: 1390001206479738368
• NII論文ID: 10031164769
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.120745
• COI: 1:STN:280:DC%2BC3svkt1Chtw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024389088
• PubMed: 23470755
• Web Site: http://id.ndl.go.jp/bib/024389088
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024389088
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.120745
• 本文言語コード: en
• データソース種別:
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  • Crossref
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