Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts

• SAKAMOTO Shingo

  Graduate School of Biosphere Sciences, Hiroshima University

• FUJIKAWA Yukichi

  Graduate School of Biosphere Sciences, Hiroshima University

• ESAKA Muneharu

  Graduate School of Biosphere Sciences, Hiroshima University

Abstract

We established a new method of transient expression using tomato fruit protoplasts. The method showed that L-ascorbic acid (AsA) content in tomato protoplasts was increased by transient expression of the L-galactose-1-phosphate phosphatase gene. This system provides a means of rapid analysis to clarify the function of AsA biosynthetic enzymes and AsA roles in tomato fruit.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 77 (3), 673-675, 2013

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• ascorbic acid
• tomato fruit
• protoplast
• transient gene expression

Details

• CRID: 1390001206479317504
• NII Article ID: 10031164797
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.120844
• COI: 1:STN:280:DC%2BC3svkt1CitA%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 024391483
• PubMed: 23470746
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024391483
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.120844
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed