Diffusion-controlled Detection of Glucose with Microelectrodes in Mediated Bioelectrocatalytic Oxidation

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  • NODA Tatsuo
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • WANIBUCHI Mizue
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • KITAZUMI Yuki
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • TSUJIMURA Seiya
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University Division of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba
  • SHIRAI Osamu
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • YAMAMOTO Masahiro
    Department of Chemistry, Konan University
  • KANO Kenji
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

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抄録

This paper describes a diffusion-controlled electrolysis of glucose with mediated bioelectrocatalysis at microdisk-electrodes. Under conditions of an extremely fast enzyme reaction, compared with the diffusion of glucose, the current in potential-step chronoamperometry reaches an almost steady state within 10 s, and is proportional to the glucose concentration. The current can be detected at +0.1 V (vs. Ag|AgCl) with 9,10-phenanthrenequinone as a mediator. The addition of carboxymethylcellulose increased the linear range up to 10 mM.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 29 (3), 279-281, 2013

    社団法人 日本分析化学会

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