Single-Molecule Imaging of Gold-Binding Peptide Adsorbed on Au(111)

• KANATA Satoshi

  Department of Physics and Electronics, Graduate School of Engineering, Osaka Prefecture University

• NISHINO Tomoaki

  Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University

• MAKIURA Rie

  Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University

• SAIKI Sho

  Department of Physical Science, Graduate School of Science, Osaka Prefecture University

• HAYASHI Nobuhiko

  Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University

抄録

Inorganic-binding peptides, which exhibit specific binding affinity to an inorganic material, are versatile building blocks in the construction of novel bio-conjugated materials. However, very little knowledge regarding their adsorbed structures on the target material is currently available. In this article, we report on the single-molecule analysis of such polypeptides by scanning tunneling microscopy (STM). The adsorbed structure of a gold-binding peptide (GBP) on Au(111) was observed at the single-molecule level. FTIR spectroscopy revealed the helical structure of the GBP, and ab initio calculations confirmed the correlation between the observed STM image and a sample helical structure. It has been demonstrated that the conformational structure of the polypeptide is highly pre-organized, allowing favorable binding onto the gold surface. Gaining such an insight into the relation between the structure and the binding function of the peptide leads to a fundamental understanding of inorganic-binding peptide, and, consequently, to a rational design of these peptides.

収録刊行物

• Analytical Sciences

  Analytical Sciences 29 (4), 405-409, 2013

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204261672704
• NII論文ID: 10031165330
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.29.405
• COI: 1:STN:280:DC%2BC3srkt1yitQ%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 024405320
• PubMed: 23574666
• Web Site: http://id.ndl.go.jp/bib/024405320
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024405320
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.29.405.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.29.405/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/29/4/29_405/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
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