Evaluation of Pathogenicity of Candida albicans in Germination-ready States Using a Silkworm Infection Model

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Author(s)

Abstract

We previously developed an <i>N</i>-acetyl-D-glucosamine (GlcNAc) medium which induces <i>Candida albicans</i> to undergo a yeast-to-hyphal transition through a cAMP-PKA pathway. Microarray analysis demonstrated that 18 genes, including <i>ALS3</i> that encodes a cell wall adhesion, were upregulated by 30-min incubation of yeast cells at 37°C in the GlcNAc medium. To investigate the differences between morphological transition and morphotype in <i>C. albicans</i> as a consequence of infection, this study utilized a silkworm infection model as an invertebrate mini-host. We prepared 3 different conditions of <i>C. albicans</i> cells <i>in vitro</i> by changing the incubation times in the GlcNAc medium: yeast-form cells at 0 min (Y0 cells), yeast-form cells in germination-ready state at 60 min (Y60 cells), and hyphal cells at 120 min (H120 cells), and compared their pathogenicities. We performed the infection study at various temperatures to find temperature-dependent virulence factors <i>in vivo</i>. Y60 cells in germination-ready state in the GlcNAc medium showed higher pathogenicity <i>in vivo</i> compared to Y0 and H120 cells at 30°C. Y60 cells proliferated in silkworms 24 h post-injection at 30°C, whereas the other 2 cell types did not. <i>In vitro</i> analysis demonstrated that Y60 cells, but not Y0 cells, germinated in the silkworm hemolymph at 30°C. However, Y0 and Y60 cells showed a similar degree of germination in the silkworm hemolymph at 37°C, although no significant difference in silkworm survival after infection with each cell type was observed at 37°C. These results suggested that the germination-ready state induced by the GlcNAc medium contributed to virulence in the silkworm.

Journal

  • Medical Mycology Journal

    Medical Mycology Journal 54(2), 131-140, 2013-04-30

    The Japanese Society for Medical Mycology

References:  28

Codes

  • NII Article ID (NAID)
    10031166014
  • NII NACSIS-CAT ID (NCID)
    AA12518136
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    21856486
  • NDL Article ID
    024731602
  • NDL Call No.
    Z19-348
  • Data Source
    CJP  NDL  J-STAGE 
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