QCM Sensor Using Membrane Proteins of Cell-derived Liposomes

• Yamanaka Makoto

  Research Center for Bio-microsensing Technology, Kyushu Institute of Technology

• Yasuda Takashi

  Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology

Bibliographic Information

Other Title

• 細胞由来リポソーム上の膜タンパク質を利用したQCMセンサ

Abstract

This paper presents a novel QCM (quartz crystal microbalance) sensor for detecting interactions between membrane proteins and their ligands using liposomes derived from human cells. First, liposomes having BCR (B-cell receptor) were derived directly from cell membranes by stimulating human lymphocyte cells, Ramos, with NaB (sodium butyrate). Next, the liposomes were separated from the cells by filtration and micronized by sonication. Then, the liposomes were immobilized on the surface of a QCM sensor chip using cell-anchoring molecules. Several experiments demonstrated that ligand detection is achieved by frequency reduction with application of the antibody against BCR.

Journal

• IEEJ Transactions on Sensors and Micromachines

  IEEJ Transactions on Sensors and Micromachines 133 (5), 155-156, 2013

  The Institute of Electrical Engineers of Japan

Keywords

• Liposome
• Human lymphocyte
• Membrane protein
• Quartz crystal microbalance
• Ligand detection

Details

• CRID: 1390282679437790464
• NII Article ID: 10031167011
• NII Book ID: AN1052634X
• DOI: 10.1541/ieejsmas.133.155
• ISSN: 13475525
• ISSN: 13418939
• Web Site: https://www.jstage.jst.go.jp/article/ieejsmas/133/5/133_155/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed