Significant correlation between endothelial nitric oxide synthase (eNOS) expression and alveolar repair in elastase-induced rat pulmonary emphysema Significant correlation between endothelial nitric oxide synthase (eNOS) expression and alveolar repair in elastase-induced rat pulmonary emphysema

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Author(s)

    • ITO Hiromichi
    • Department of Thoracic Surgery, Graduate School of Comprehensive Human Science, University of Tsukuba
    • MATSUSHITA Shonosuke
    • Department of Thoracic Surgery, Graduate School of Comprehensive Human Science, University of Tsukuba
    • ISHIKAWA Shigemi
    • Department of Chest Surgery, International University of Health and Welfare Hospital
    • GOTO Yukinobu
    • Department of Thoracic Surgery, Graduate School of Comprehensive Human Science, University of Tsukuba
    • SAKAI Mitsuaki
    • Department of Thoracic Surgery, Graduate School of Comprehensive Human Science, University of Tsukuba
    • ONIZUKA Masataka
    • Department of Thoracic Surgery, Graduate School of Comprehensive Human Science, University of Tsukuba
    • SATO Yukio
    • Department of Thoracic Surgery, Graduate School of Comprehensive Human Science, University of Tsukuba
    • SAKAKIBARA Yuzuru
    • Department of Thoracic Surgery, Graduate School of Comprehensive Human Science, University of Tsukuba

Abstract

Background and purposeAngiogenic factors, such as endothelial nitric oxide synthase (eNOS), are thought to play an important role in the repair of pulmonary emphysema (PE); yet, the correlation of the factors involved has not been investigated. We conducted this study to clarify the positive correlation between eNOS expression and alveolar repair in PE recovery.MethodsWe used elastase to induce PE in rats, which were divided into Groups A (Control), B (G-CSF), C (PE) and D (PE + G-CSF). G-CSF was injected for 12 days, 4 weeks after which the alveolar walls, arterioles, and angiogenic factors including eNOS were examined histopathologically and by western blotting.ResultsIn comparing Groups A, B, C, and D, the alveolar density was 2.4 ± 0.2, 2.4 ± 0.1, 1.8 ± 0.1, 2.5 ± 0.1 per 100 μm2, respectively (C vs. others; p < 0.00001) and the number of arterioles was 4.5 ± 1.0, 5.6 ± 0.6, 3.2 ± 0.5, 5.5 ± 0.7/mm2, respectively (C vs. others; p < 0.05). Immunohistochemical staining (IHC) revealed different eNOS expression in Group D versus Group C (p < 0.0001) and western blotting revealed different eNOS, VEGF, and FLT-1 expression in Group D versus Group C (p < 0.01, p < 0.05, p < 0.001), reflecting the contribution of angiogenesis to PE repair. eNOS showed a significantly positive correlation to alveolar density and arteriole repair.ConclusionAlveolar repair was correlated positively with eNOS expression by vascular regeneration in elastase-induced rat PE.

Journal

  • Surgery today

    Surgery today 43(3), 293-299, 2013-03-01

    Springer-Verlag

References:  23

Codes

  • NII Article ID (NAID)
    10031168006
  • NII NACSIS-CAT ID (NCID)
    AA10824685
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09411291
  • Data Source
    CJP  IR 
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