The Effects of Adjuvants on Autoimmune Responses Against Testicular Antigens in Mice
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- MUSHA Muhetaerjiang
- Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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- HIRAI Shuichi
- Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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- NAITO Munekazu
- Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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- TERAYAMA Hayato
- Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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- QU Ning
- Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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- HATAYAMA Naoyuki
- Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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- ITOH Masahiro
- Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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Experimental autoimmune orchitis (EAO) is a model of immunologic male infertility and pathologically characterized by lymphocytic inflammation, which causes breakdown of the testicular immune privilege with spermatogenic disturbance. Generally, murine EAO is induced by immunization with testicular homogenate (TH) from the testes of donor mice + complete Freund’s adjuvant (CFA) + Bordetella pertussigens (BP), and it has been considered that treatment with these two adjuvants is required to enhance the immune response against testicular antigens. However, there remains a possibility that CFA and BP may affect autoimmune responses against the testicular antigens without TH. In the present study, we examined this possibility using real-time RT-PCR, Western blotting and immunohistochemical staining. The results demonstrated that immunization with TH in combination with CFA and BP evoked more severe EAO than that with only TH. Real-time RT-PCR analyses revealed that Fas mRNA expression in TH+CFA+BP-induced EAO was significantly higher than that in TH-induced EAO. Interestingly, IL-6 mRNA expression dramatically increased in TH+CFA+BP-induced EAO; however, no apparent change in IL-6 mRNA expression occurred in TH-induced EAO. It was also noted that treatment with CFA and BP alone augmented autoimmune reactions against some testicular autoantigens. These results indicates that these adjuvants are helpful in evoking severe EAO, and treatment with the adjuvants alone can evoke autoimmune reactions against some testicular autoantigens despite the use of no TH.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 59 (2), 139-144, 2013
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206335083648
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- NII論文ID
- 10031170771
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC3s3jslSjsg%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 024426198
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- PubMed
- 23257732
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可