A Novel Mouse Model of Chronic Inflammatory and Overactive Bladder by a Single Intravesical Injection of Hydrogen Peroxide

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Author(s)

Abstract

There is so far no generally accepted animal model of chronic cystitis by which potential therapies can be evaluated. In this study, we aimed to establish a new mouse model of cystitis based on the proinflammatory effects of reactive oxygen species. A single intravesical injection of 1.5% hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) significantly increased the numbers of voids by 1 day after injection in female mice, which lasted up to 7 days. The H<sub>2</sub>O<sub>2</sub> injection rapidly increased the bladder weight by 3 h in parallel with the histological damage and hyperpermeability of urothelial barrier. Although the urothelial dysfunction was recovered to normal by 7 days, increase in bladder weight, edematous thickening of the submucosa, and vascular hyperpermeability were apparent even 7 days after injection. During the time course, massive infiltration of neutrophils and increased expression of inflammatory cytokines were observed in the bladder. An intraperitoneal administration of oxybutynin, amitriptyline, indomethacin, or morphine attenuated the H<sub>2</sub>O<sub>2</sub>-induced frequent urination. These findings suggest that an intravesical injection of H<sub>2</sub>O<sub>2</sub> induces relatively long-lasting inflammatory and overactive bladder, compared with existing cystitis models. The intravesical H<sub>2</sub>O<sub>2</sub> injection model may be a simple and useful tool in the pathological study and drug discovery for chronic cystitis.

Journal

  • Journal of Pharmacological Sciences

    Journal of Pharmacological Sciences 121(4), 327-337, 2013-04-20

    The Japanese Pharmacological Society

References:  58

Codes

  • NII Article ID (NAID)
    10031171143
  • NII NACSIS-CAT ID (NCID)
    AA11806667
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13478613
  • NDL Article ID
    024434347
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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