A Novel Mouse Model of Chronic Inflammatory and Overactive Bladder by a Single Intravesical Injection of Hydrogen Peroxide
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- Homan Takashi
- Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan Discovery Research Laboratories, Nippon Shinyaku Co., Ltd., Japan
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- Tsuzuki Tetsunori
- Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
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- Dogishi Koji
- Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
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- Shirakawa Hisashi
- Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
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- Oyama Tatsuya
- Discovery Research Laboratories, Nippon Shinyaku Co., Ltd., Japan
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- Nakagawa Takayuki
- Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
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- Kaneko Shuji
- Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
Bibliographic Information
- Other Title
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- Novel mouse model of chronic inflammatory and overactive bladder by a single intravesical injection of hydrogen peroxide
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Abstract
There is so far no generally accepted animal model of chronic cystitis by which potential therapies can be evaluated. In this study, we aimed to establish a new mouse model of cystitis based on the proinflammatory effects of reactive oxygen species. A single intravesical injection of 1.5% hydrogen peroxide (H2O2) significantly increased the numbers of voids by 1 day after injection in female mice, which lasted up to 7 days. The H2O2 injection rapidly increased the bladder weight by 3 h in parallel with the histological damage and hyperpermeability of urothelial barrier. Although the urothelial dysfunction was recovered to normal by 7 days, increase in bladder weight, edematous thickening of the submucosa, and vascular hyperpermeability were apparent even 7 days after injection. During the time course, massive infiltration of neutrophils and increased expression of inflammatory cytokines were observed in the bladder. An intraperitoneal administration of oxybutynin, amitriptyline, indomethacin, or morphine attenuated the H2O2-induced frequent urination. These findings suggest that an intravesical injection of H2O2 induces relatively long-lasting inflammatory and overactive bladder, compared with existing cystitis models. The intravesical H2O2 injection model may be a simple and useful tool in the pathological study and drug discovery for chronic cystitis.
Journal
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 121 (4), 327-337, 2013
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390282680156031232
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- NII Article ID
- 10031171143
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- NII Book ID
- AA11806667
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- COI
- 1:CAS:528:DC%2BC3sXnt1aisL0%3D
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- ISSN
- 13478648
- 13478613
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- NDL BIB ID
- 024434347
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- PubMed
- 23545478
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed