Development of the Separation-oriented Derivatization Method in HPLC Analysis Using Pentafluorophenyl Moiety

• KAWAMI Yusuke

  Faculty of Pharmaceutical Sciences, Fukuoka University

• SAKAGUCHI Yohei

  Faculty of Pharmaceutical Sciences, Fukuoka University

• YOSHIDA Hideyuki

  Faculty of Pharmaceutical Sciences, Fukuoka University

• TAMASHIMA Erina

  Faculty of Pharmaceutical Sciences, Fukuoka University

• ITOYAMA Miki

  Faculty of Pharmaceutical Sciences, Fukuoka University

• HAYAMA Tadashi

  Faculty of Pharmaceutical Sciences, Fukuoka University

• TODOROKI Kenichiro

  School of Pharmaceutical Sciences, University of Shizuoka

• YAMAGUCHI Masatoshi

  Faculty of Pharmaceutical Sciences, Fukuoka University

• NOHTA Hitoshi

  Faculty of Pharmaceutical Sciences, Fukuoka University

Bibliographic Information

Other Title

• ペンタフルオロフェニル構造を用いる分離指向性誘導体化−HPLC分析法の開発
• ペンタフルオロフェニル構造を用いる分離指向性誘導体化-HPLC分析法の開発
• ペンタフルオロフェニル コウゾウ オ モチイル ブンリ シコウセイ ユウドウタイカ-HPLC ブンセキホウ ノ カイハツ

Abstract

We have developed a new separation-oriented derivatization method based on the affinity of the interaction between the pentafluorophenyl (PFP) group for the HPLC determination of native fluorescent non-steroidal anti-inflammatory drugs (NSAIDs). This method involves the derivatization of NSAIDs with pentafluorobenzyl bromide and the separation of derivatives using a PFP-modified stationary phase column. In this method, the PFP-derivatized NSAIDs were selectively retained on the PFP column, followed by fluorescence detection, whereas the derivatives were not retained on the ODS-, phenyl-, and perfluoroalkyl-modified HPLC columns. The detection limits (signal-to-noise ratio of 3) of the NSAID standards were found to be 44 – 84 fmol on column for a 20 μL injection, and the repeatability of this method was within 7.1% as relative standard deviation. We show that the proposed method can be used to analyze NSAIDs in human plasma samples without the interference of endogenous matrices.

Journal

• BUNSEKI KAGAKU

  BUNSEKI KAGAKU 62 (8), 713-717, 2013

  The Japan Society for Analytical Chemistry

Keywords

• separation-oriented derivatization
• HPLC
• pentafluorophenyl group
• NSAIDs

Details

• CRID: 1390001204661494144
• NII Article ID: 10031178769
• NII Book ID: AN00222633
• DOI: 10.2116/bunsekikagaku.62.713
• COI: 1:CAS:528:DC%2BC3sXhsVWiu7%2FK
• NDL BIB ID: 024835360
• ISSN: 05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024835360
• Web Site: https://www.jstage.jst.go.jp/article/bunsekikagaku/62/8/62_713/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed