Determination of Cortisol and Cortisone in Human Saliva Using Dispersive Liquid-liquid Microextraction Coupled to HPLC Analysis

• TAMASHIMA Erina

  Faculty of Pharmaceutical Sciences, Fukuoka University

• KAI Tomomi

  Faculty of Pharmaceutical Sciences, Fukuoka University

• TODOROKI Kenichiro

  School of Pharmaceutical Sciences, University of Shizuoka

• KAWAMI Yusuke

  Faculty of Pharmaceutical Sciences, Fukuoka University

• ITOYAMA Miki

  Faculty of Pharmaceutical Sciences, Fukuoka University

• HAYAMA Tadashi

  Faculty of Pharmaceutical Sciences, Fukuoka University

• YOSHIDA Hideyuki

  Faculty of Pharmaceutical Sciences, Fukuoka University

• YAMAGUCHI Masatoshi

  Faculty of Pharmaceutical Sciences, Fukuoka University

• NOHTA Hitoshi

  Faculty of Pharmaceutical Sciences, Fukuoka University

Bibliographic Information

Other Title

• ヒト唾液中コルチゾール及びコルチゾンの分散液液マイクロ抽出/HPLC分析
• ヒト ダエキ チュウ コルチゾール オヨビ コルチゾン ノ ブンサンエキエキ マイクロ チュウシュツ/HPLC ブンセキ

Abstract

We examined the determination of cortisol and cortisone in human saliva with dispersive liquid-liquid microextraction (DLLME) coupled to HPLC/UV detection. In this study, by adding a mixture of acetonitrile (disperser solvent) and 1,1,2,2-tetrachloroethane (extraction solvent) into the sample, DLLME was performed. The resulting sedimented phase was injected into a reversed-phase HPLC column using a mixture of water, acetonitrile, and trifluoroacetic acid as a mobile phase for isocratic elution, and the analytes were determined with an ultraviolet detector at 254 nm. The enrichment factors of this method were ca. 20 and 40 for cortisol and cortisone, respectively, and the limits of detection were 0.30 ng mL⁻¹ and 0.20 ng mL⁻¹, respectively. We showed that the proposed method can be used to analyze trace amounts of cortisone in human saliva samples with sufficient sensitivity.

Journal

• BUNSEKI KAGAKU

  BUNSEKI KAGAKU 62 (8), 719-723, 2013

  The Japan Society for Analytical Chemistry

Keywords

• cortisol
• cortisone
• DLLME
• human saliva

Details

• CRID: 1390282679638203520
• NII Article ID: 10031178770
• NII Book ID: AN00222633
• DOI: 10.2116/bunsekikagaku.62.719
• COI: 1:CAS:528:DC%2BC3sXhsVWiu7%2FL
• NDL BIB ID: 024835383
• ISSN: 05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024835383
• Web Site: https://www.jstage.jst.go.jp/article/bunsekikagaku/62/8/62_719/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013353049
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed