Biogenesis of trans-acting siRNAs, endogenous secondary siRNAs in plants

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Author(s)

Abstract

<i>Trans</i>-acting small interfering RNAs (tasiRNAs) are plant-specific endogenous siRNAs that control non-identical mRNAs via cleavage. The production of tasiRNAs is triggered by cleavage of capped and polyadenylated primary <i>TAS</i> transcripts (pri-TASs) by specific miRNAs. Following miRNA-directed cleavage, either 5′ or 3′ cleavage fragments are converted into double-stranded RNAs (dsRNAs) by RNA-DEPENDENT RNA POLYMERASE 6. The dsRNAs are processed to tasiRNAs by DICER-LIKE 4 in a phasing manner. There are two forms of pri-TASs; One has a single miRNA target site that is targeted by 22-nucleotide microRNAs, and the other has two miR390 target sites. Secondary siRNAs that are important for the amplification of RNA silencing are defined as siRNAs whose production is initiated by the cleavage of primary small RNA-containing RNA-induced silencing complexes. Thus, tasiRNA production is a model system of secondary siRNA production in plants. This review focuses on the production of tasiRNAs that are endogenous secondary siRNAs.

Journal

  • Genes & Genetic Systems

    Genes & Genetic Systems 88(2), 77-84, 2013-04-25

    The Genetics Society of Japan

References:  69

Codes

  • NII Article ID (NAID)
    10031183173
  • NII NACSIS-CAT ID (NCID)
    AA11077421
  • Text Lang
    ENG
  • Article Type
    REV
  • ISSN
    13417568
  • NDL Article ID
    024638433
  • NDL Call No.
    Z53-W539
  • Data Source
    CJP  NDL  J-STAGE 
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