Characterization of 5-Hydroxyconiferaldehyde O-Methyltransferase in Oryza sativa

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Abstract

Little is known regarding syringyl lignin biosynthesis in rice (<i>Oryza sativa</i> L. cv. Nipponbare). In the present study, the role of rice caffeic acid <i>O</i>-methyltransferase (OsCOMT1, Q6ZD89), was examined. The recombinant OsCOMT1 catalyzed the 5-<i>O</i>methylation of 5-hydroxyferulate (5-HFA) and 5-hydroxyconiferaldehyde (5-HCAld). 5-HCAld inhibited 5-HFA methylation by this <i>O</i>-methyltransferase (OMT), while 5-HFA mitigated self-inhibition in 5-HCAld methylation. A rice plant in which <i>OsCOMT1</i> expression was downregulated exhibited weakened cell wall staining with Wiesner reagent in vascular bundle cells and sclerenchyma tissue, compared with wild-type plants. The lignin content of transgenic rice plants was decreased and the syringyl lignin content reduced largely compared with that of the wild type. Taken together, these data indicated that OsCOMT1 functioned as a 5-HCAld OMT (OsCAldOMT1) in the biosynthetic pathway to syringyl lignin.

Journal

  • Plant Biotechnology

    Plant Biotechnology 30(2), 157-167, 2013-06-25

    Japanese Society for Plant Cell and Molecular Biology

References:  39

Codes

  • NII Article ID (NAID)
    10031184120
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13424580
  • NDL Article ID
    024676595
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  NDL  J-STAGE 
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