Ghrelin counteracts salt-induced hypertension via promoting diuresis and renal nitric oxide production in Dahl rats

• Aoki Hirotaka

  Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan Depertment of Internal medicine, Division of Cardiovascular Medicine, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan

• Nakata Masanori

  Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan

• Dezaki Katsuya

  Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan

• Lu Ming

  Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan

• Gantulga Darambazar

  Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan

• Yamamoto Keiji

  Depertment of Internal medicine, Division of Cardiovascular Medicine, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan Department of General Internal Medicine, Saitama Medical School, Saitama 350-0495, Japan

• Shimada Kazuyuki

  Depertment of Internal medicine, Division of Cardiovascular Medicine, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan

• Kario Kazuomi

  Depertment of Internal medicine, Division of Cardiovascular Medicine, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan

• Yada Toshihiko

  Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, Shimotsuke 329-0498, Japan

Abstract

Ghrelin is the endogenous ligand for the growth hormone-secretagogue receptor expressed in various tissues including the heart, blood vessels and kidney. This study sought to determine the effects of long-term treatment with ghrelin (10 nmol/kg, twice a day, intraperitoneally) on the hypertension induced by high salt (8.0% NaCl) diet in Dahl salt-sensitive hypertensive (DS) rats. Systolic blood pressure (SBP) was measured by a tail cuff method. During the treatment period for 3 weeks, high salt diet increased blood pressure compared to normal salt (0.3% NaCl) diet, and this hypertension was partly but significantly (P<0.01) attenuated by simultaneous treatment with ghrelin. Ghrelin significantly increased urine volume and tended to increase urine Na⁺ excretion. Furthermore, ghrelin increased urine nitric oxide (NO) excretion and tended to increase renal neuronal nitric oxide synthase (nNOS) mRNA expression. Ghrelin did not alter the plasma angiotensin II level and renin activity, nor urine catecholamine levels. Furthermore, ghrelin prevented the high salt-induced increases in heart thickness and plasma ANP mRNA expression. These results demonstrate that long-term ghrelin treatment counteracts salt-induced hypertension in DS rats primarily through diuretic action associated with increased renal NO production, thereby exerting cardio-protective effects.

Journal

• Endocrine Journal

  Endocrine Journal 60 (5), 571-581, 2013

  The Japan Endocrine Society

Keywords

• Salt-sensitive hypertension
• Ghrelin
• Nitric oxide
• Diuresis
• Cardiac protection

Details

• CRID: 1390001206299432320
• NII Article ID: 10031184524
• NII Book ID: AA10901436
• DOI: 10.1507/endocrj.ej12-0371
• COI: 1:STN:280:DC%2BC3szgsVamsw%3D%3D
• ISSN: 13484540
• ISSN: 09188959
• PubMed: 23328675
• Web Site: https://www.jstage.jst.go.jp/article/endocrj/60/5/60_EJ12-0371/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014196798
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed